新型长链非编码 RNA AK023507 通过 β-连环蛋白/Wnt 信号通路抑制甲状腺乳头状癌细胞的转移和增殖。
Novel LncRNA AK023507 inhibits cell metastasis and proliferation in Papillary Thyroid Cancer through β-catenin/Wnt Signaling Pathway.
机构信息
Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, China.
The First Affiliated Hospital of Wenzhou Medical University, China.
出版信息
Biochem Biophys Res Commun. 2023 May 7;655:104-109. doi: 10.1016/j.bbrc.2023.03.033. Epub 2023 Mar 14.
INTRODUCTION
Papillary Thyroid Cancer (PTC) represents a commonly encountered type of thyroid malignancy whose occurrence and development is influenced by long non-coding RNA (LncRNA). A novel lncRNA (LncRNA AK023507), known to have tumor suppressive functions, was shown to prevent breast cancer cells from proliferating and metastasizing, but its mechanism in PTC is unclear.
METHODS
Using PTC tissues and cell lines, the expression of LncRNA AK023507 was investigated by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). The effects of knockdown or overexpression of LncRNA AK023507 on cell growth and movement were investigated through various cell experiments in vitro. The presence of important functional proteins was determined by Western blotting, with the recovery experiment used for verification.
RESULTS
LncRNA AK023507 was found to have low expression in both the PTC cell lines and tissue samples. Knockdown of LncRNA AK023507 in PTC cells significantly promoted cell proliferation, migration, and invasion, while overexpression of LncRNA AK023507 resulted in the opposite effects. Furthermore, LncRNA AK023507 could regulate the expression of β-catenin/Wnt signaling pathway as confirmed by recovery experiment.
CONCLUSION
By acting through the β-catenin/Wnt signaling pathway, LncRNA AK023507 prevented PTC cells from proliferating and metastasizing. These novel findings indicate that LncRNA AK023507 could be of prognostic and diagnostic value as a potential biomarker of PTC.
简介
甲状腺乳头状癌（PTC）是一种常见的甲状腺恶性肿瘤，其发生和发展受长链非编码 RNA（LncRNA）的影响。一种新的 LncRNA（LncRNA AK023507），已知具有肿瘤抑制功能，可防止乳腺癌细胞增殖和转移，但在 PTC 中的机制尚不清楚。
方法
通过定量实时聚合酶链反应（qRT-PCR）检测 PTC 组织和细胞系中 LncRNA AK023507 的表达。通过体外各种细胞实验研究 LncRNA AK023507 敲低或过表达对细胞生长和运动的影响。通过 Western blot 确定重要功能蛋白的存在，并通过恢复实验进行验证。
结果
LncRNA AK023507 在 PTC 细胞系和组织样本中的表达均较低。PTC 细胞中 LncRNA AK023507 的敲低显著促进了细胞增殖、迁移和侵袭，而过表达 LncRNA AK023507 则产生相反的效果。此外，通过恢复实验证实 LncRNA AK023507 可以调节 β-连环蛋白/Wnt 信号通路的表达。
结论
通过作用于β-连环蛋白/Wnt 信号通路，LncRNA AK023507 阻止 PTC 细胞增殖和转移。这些新发现表明，LncRNA AK023507 可能作为 PTC 的潜在生物标志物具有预后和诊断价值。
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