Horiuchi Shinya, Yumoto Hiromichi, Kimura Tomoko, Sato Minami, Mitsui Silvia Naomi, Hiasa Masahiro, Nishikawa Eiji, El-Bialy Tarek, Tanaka Eiji
Department of Orthodontics and Dentofacial Orthopedics, Tokushima University Hospital, Tokushima, Japan.
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Hospital, Tokushima, Japan.
Am J Dent. 2018 Apr;31(2):67-70.
To evaluate the effect of pulsed ultrasound toothbrush on the removal of biofilm formed by Streptococcus mutans (S. mutans).
S. mutans biofilm grown on apatite pellet was destructed with four different sonic action toothbrushes: 1) pulsed ultrasound with sonic vibration (PUV); 2) continuous ultrasound with sonic vibration (CUV); 3) sonic vibration only (SV); and 4) no ultrasound nor sonic vibration (control). After 3 minutes of noncontact brushing, the amount of water-insoluble glucan was measured, and the residual biofilm was observed by scanning electron microscopy.
PUV group revealed the smallest amount of the residual water-insoluble glucans (32 ± 19%), followed by the CUV group (54 ± 12%) and the SV group (64 ± 13%). The PUV group showed a significantly lower amount of the residual water-insoluble glucan than the SV group, while no significant difference was found between SV and CUV. The bacterial adherence and aggregation notably decreased in the PUV group, compared to the remaining three groups.
The sonic vibration with pulsed ultrasound showed more reduction of the biofilm compared to the control and the sonic vibration with and without continuous ultrasound. Thus, pulsed ultrasound action may be beneficial for biofilm removal of interproximal regions.
评估脉冲超声牙刷对变形链球菌形成的生物膜的清除效果。
用四种不同的声波作用牙刷破坏在磷灰石颗粒上生长的变形链球菌生物膜：1）脉冲超声加声波振动（PUV）；2）连续超声加声波振动（CUV）；3）仅声波振动（SV）；4）无超声和声波振动（对照）。非接触刷牙3分钟后，测量水不溶性葡聚糖的量，并通过扫描电子显微镜观察残留生物膜。
PUV组残留的水不溶性葡聚糖量最少（32±19%），其次是CUV组（54±12%）和SV组（64±13%）。PUV组残留的水不溶性葡聚糖量显著低于SV组，而SV组和CUV组之间未发现显著差异。与其他三组相比，PUV组的细菌黏附和聚集明显减少。
与对照组以及有和没有连续超声的声波振动相比，脉冲超声加声波振动显示出对生物膜的清除效果更佳。因此，脉冲超声作用可能有利于清除邻面区域的生物膜。
