Mersin University, Faculty of Pharmacy, Department of Toxicology, Mersin, Turkey; Near East University, Faculty of Pharmacy, Department of Toxicology, Nicosia, Cyprus.
Hacettepe University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
Biosens Bioelectron. 2018 Jul 15;111:10-17. doi: 10.1016/j.bios.2018.03.055. Epub 2018 Mar 28.
Herein, we prepared a novel quartz crystal microbalance (QCM) sensor for synthetic cannabinoids (JWH-073, JWH-073 butanoic acid, JWH-018 and JWH-018 pentanoic acid,) detection. Firstly, the synthetic cannabinoid (SCs) imprinted (MIP) and non-imprinted (NIP) nanoparticles were synthesized by mini-emulsion polymerization system. The SCs-imprinted nanoparticles were first characterized by SEM, TEM, zeta-size and FTIR-ATR analysis and then were dropped onto the gold QCM surface. The SCs-imprinted QCM sensor was characterized by an ellipsometer, contact angle, and AFM. The limit of detection was found as 0.3, 0.45, 0.4, 0.2 pg/mL JWH-018, JWH-073, JWH-018 pentanoic acid and JWH-073 butanoic acid, respectively. The selectivity of the SCs-imprinted QCM sensor was shown by using JWH-018, JWH-018 pentanoic acid, JWH-073 and JWH-073 butanoic acid. According to the results, the SCs-imprinted QCM sensors show highly selective and sensitive in a broad range of synthetic cannabinoid concentrations (0.0005-1.0 ng/mL) in both aqueous and synthetic urine solutions.
本文制备了一种新型石英晶体微天平（QCM）传感器，用于检测合成大麻素（JWH-073、JWH-073 丁酸、JWH-018 和 JWH-018 戊酸）。首先，通过微乳液聚合体系合成了合成大麻素（SCs）印迹（MIP）和非印迹（NIP）纳米粒子。SC 印迹纳米粒子首先通过 SEM、TEM、Zeta 尺寸和 FTIR-ATR 分析进行表征，然后滴到金 QCM 表面。SC 印迹 QCM 传感器通过椭圆偏光仪、接触角和 AFM 进行表征。检测限分别为 0.3、0.45、0.4 和 0.2 pg/mL 的 JWH-018、JWH-073、JWH-018 戊酸和 JWH-073 丁酸。通过使用 JWH-018、JWH-018 戊酸、JWH-073 和 JWH-073 丁酸对 SC 印迹 QCM 传感器的选择性进行了研究。结果表明，SC 印迹 QCM 传感器在水相和合成尿液溶液中均能在 0.0005-1.0 ng/mL 的宽合成大麻素浓度范围内表现出高度选择性和灵敏性。
