Eren Tanju, Atar Necip, Yola Mehmet Lütfi, Karimi-Maleh Hassan
Pamukkale University, Department of Chemical Engineering, Denizli, Turkey.
Pamukkale University, Department of Chemical Engineering, Denizli, Turkey.
Food Chem. 2015 Oct 15;185:430-6. doi: 10.1016/j.foodchem.2015.03.153. Epub 2015 Apr 11.
Lovastatin (LOV) is a statin, used to lower cholesterol which has been found as a hypolipidemic agent in commercial red yeast rice. In present study, a sensitive molecular imprinted quartz crystal microbalance (QCM) sensor was prepared by fabricating a self-assembling monolayer formation of allylmercaptane on QCM chip surface for selective determination of lovastatin (LOV) in red yeast rice. To prepare molecular imprinted quartz crystal microbalance (QCM) nanosensor, LOV imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoaspartic acid) [p(HEMA-MAAsp)] nanofilm was attached on the modified gold surface of QCM chip. The non-modified and improved surfaces were characterized by using contact angle, atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy. The imprinted QCM sensor was validated according to the ICH guideline (International Conference on Harmonisation). The linearity range was obtained as 0.10-1.25 nM. The detection limit of the prepared material was calculated as 0.030 nM. The developed QCM nanosensor was successfully used to examine red yeast rice. Furthermore, the stability and repeatability of the prepared QCM nanosensor were studied. The spectacular long-term stability and repeatability of the prepared LOV-imprinted QCM nanosensor make them intriguing for use in QCM sensors.
洛伐他汀（LOV）是一种他汀类药物，用于降低胆固醇，已在商业化的红曲米中被发现是一种降血脂剂。在本研究中，通过在石英晶体微天平（QCM）芯片表面制备烯丙基硫醇的自组装单分子层，制备了一种灵敏的分子印迹石英晶体微天平（QCM）传感器，用于选择性测定红曲米中的洛伐他汀（LOV）。为制备分子印迹石英晶体微天平（QCM）纳米传感器，将LOV印迹的聚（甲基丙烯酸2-羟乙酯-甲基丙烯酰氨基天冬氨酸）[p（HEMA-MAAsp）]纳米膜附着在QCM芯片的修饰金表面上。使用接触角、原子力显微镜（AFM）和傅里叶变换红外（FTIR）光谱对未修饰和改进后的表面进行了表征。根据国际协调会议（ICH）指南对印迹QCM传感器进行了验证。线性范围为0.10-1.25 nM。所制备材料的检测限计算为0.030 nM。所开发的QCM纳米传感器成功用于检测红曲米。此外，还研究了所制备的QCM纳米传感器的稳定性和重复性。所制备的LOV印迹QCM纳米传感器具有出色的长期稳定性和重复性使其在QCM传感器中具有吸引力。
