Chair of Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Germany.
Bioresour Technol. 2018 Jul;260:302-310. doi: 10.1016/j.biortech.2018.03.113. Epub 2018 Mar 30.
In the biorefinery concept renewable feedstocks are converted to a multitude of value-added compounds irrespective of seasonal or other variations of the complex biomass substrates. Conceptionally, this can be realized by specialized single microbial strains or by co-culturing various strain combinations. In the latter approach strains for substrate conversion and for product formation can be combined. This study addressed the construction of binary microbial consortia based on starch- and sucrose-based production of l-lysine and derived value-added compounds. A commensalism-based synthetic consortium for l-lysine production from sucrose was developed combining an l-lysine auxotrophic, naturally sucrose-negative E. coli strain with a C. glutamicum strain able to produce l-lysine that secretes fructose when grown with sucrose due to deletion of the fructose importer gene ptsF. Mutualistic synthetic consortia with an l-lysine auxotrophic, α-amylase secreting E. coli strain and naturally amylase-negative C. glutamicum strains was implemented for production of valuable fine chemicals from starch.
在生物炼制概念中，可再生原料被转化为多种增值化合物，而不受复杂生物质基质的季节性或其他变化的影响。从概念上讲，这可以通过专门的单一微生物菌株或共培养各种菌株组合来实现。在后一种方法中，可以将用于底物转化和产物形成的菌株组合在一起。本研究基于淀粉和蔗糖生产 l-赖氨酸和衍生的增值化合物，构建了基于二元微生物共生体。从蔗糖生产 l-赖氨酸的共生合成共合体是通过将 l-赖氨酸营养缺陷型、天然蔗糖阴性大肠杆菌菌株与能够生产 l-赖氨酸的谷氨酸棒杆菌菌株结合而开发的，该菌株由于缺失果糖转运蛋白基因 ptsF，当与蔗糖一起生长时会分泌果糖。对于从淀粉生产有价值的精细化学品，构建了具有 l-赖氨酸营养缺陷型、α-淀粉酶分泌大肠杆菌菌株和天然淀粉酶阴性谷氨酸棒杆菌菌株的互利共生合成共合体。
