Yan Wenlong, Cao Zhibei, Ding Mingzhu, Yuan Yingjin
Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, China.
Synth Syst Biotechnol. 2022 Nov 18;8(1):176-185. doi: 10.1016/j.synbio.2022.11.001. eCollection 2023 Mar.
Environmental sustainability is an increasingly important issue in industry. As an environmentally friendly and sustainable way, constructing microbial cell factories to produce all kinds of valuable products has attracted more and more attention. In the process of constructing microbial cell factories, systems biology plays a crucial role. This review summarizes the recent applications of systems biology in the design and construction of microbial cell factories from four perspectives, including functional genes/enzymes discovery, bottleneck pathways identification, strains tolerance improvement and design and construction of synthetic microbial consortia. Systems biology tools can be employed to identify functional genes/enzymes involved in the biosynthetic pathways of products. These discovered genes are introduced into appropriate chassis strains to build engineering microorganisms capable of producing products. Subsequently, systems biology tools are used to identify bottleneck pathways, improve strains tolerance and guide design and construction of synthetic microbial consortia, resulting in increasing the yield of engineered strains and constructing microbial cell factories successfully.
环境可持续性在工业中是一个日益重要的问题。作为一种环境友好且可持续的方式，构建微生物细胞工厂来生产各种有价值的产品已受到越来越多的关注。在构建微生物细胞工厂的过程中，系统生物学起着至关重要的作用。本综述从四个方面总结了系统生物学在微生物细胞工厂设计与构建中的最新应用，包括功能基因/酶的发现、瓶颈途径的识别、菌株耐受性的提高以及合成微生物群落的设计与构建。系统生物学工具可用于识别参与产品生物合成途径的功能基因/酶。将这些发现的基因导入合适的底盘菌株中，以构建能够生产产品的工程微生物。随后，利用系统生物学工具识别瓶颈途径、提高菌株耐受性并指导合成微生物群落的设计与构建，从而提高工程菌株的产量并成功构建微生物细胞工厂。
