Buckley K J, Hayashi M
Department of Biology, University of California, San Diego, La Jolla 92093.
J Mol Biol. 1987 Dec 20;198(4):599-607. doi: 10.1016/0022-2836(87)90203-8.
Expression of the phi X174 lysis (E) gene, a member of an overlapping gene pair, appears to depend on a frameshift-induced chain termination by ribosomes translating the upstream D gene. A -1 reading frameshift, possibly induced by misreading of an alanine codon as a doublet, causes ribosomes to terminate translation at two different sites, suggesting two modes of regulating expression of the E gene. One frameshift can cause translational termination at a stop codon(s) near the E gene ribosome binding site (RBS), resulting in reinitiation by ribosomes at the E gene RBS. Termination at a second site some 70 bases upstream from the E gene RBS, while too far away to allow ribosomal re-initiation at the E gene RBS, probably results in an unmasking of the message, allowing entry of a new ribosome at the E gene RBS.
φX174裂解（E）基因是一对重叠基因中的一个成员，其表达似乎取决于核糖体在翻译上游D基因时发生的移码诱导的链终止。一个-1读码框移码，可能是由于丙氨酸密码子被误读为双峰密码子而诱导产生，导致核糖体在两个不同位点终止翻译，这表明存在两种调节E基因表达的模式。一种移码可导致在E基因核糖体结合位点（RBS）附近的一个终止密码子处发生翻译终止，从而使核糖体在E基因RBS处重新起始翻译。在E基因RBS上游约70个碱基处的第二个位点终止翻译，虽然距离太远以至于核糖体无法在E基因RBS处重新起始翻译，但可能会使信息解蔽，从而允许新的核糖体在E基因RBS处进入。
