一种隐性tRNA抑制子对-1移码突变的抑制，该抑制子导致双联体解码。
Suppression of a -1 frameshift mutation by a recessive tRNA suppressor which causes doublet decoding.
作者信息
O'Mahony D J, Hughes D, Thompson S, Atkins J F
机构信息
Department of Genetics, Trinity College, Dublin, Ireland.
出版信息
J Bacteriol. 1989 Jul;171(7):3824-30. doi: 10.1128/jb.171.7.3824-3830.1989.
Abstract
sufS was found to suppress the only known suppressible-1 frameshift mutation, trpE91, at a site identified as GGA and mapped within the single gene of the only tRNA that can decode GGA in Escherichia coli. It mapped to the same gene in Salmonella typhimurium. sufS alleles were recessive, and dominant alleles could not be isolated. This is in contrast to all other tRNA structural gene mutations identified thus far that cause frameshift suppression. The recessiveness implies that all sufS alleles are poor competitors against their wild-type tRNA(Gly2) counterparts. The base G immediately 5' of the GGA suppression site influenced the level but was not critical for suppression by sufS601. From this result, it is inferred that sufS601 causes frameshifting by doublet decoding.
摘要
sufS被发现可抑制唯一已知的可抑制-1移码突变trpE91，其抑制位点被确定为GGA，且位于大肠杆菌中唯一能解码GGA的tRNA的单个基因内。在鼠伤寒沙门氏菌中，它映射到同一个基因。sufS等位基因是隐性的，无法分离出显性等位基因。这与迄今为止鉴定出的所有导致移码抑制的其他tRNA结构基因突变形成对比。隐性意味着所有sufS等位基因与其野生型tRNA(Gly2)对应物相比，竞争能力较差。GGA抑制位点5'端紧邻的碱基G影响抑制水平，但对于sufS601的抑制作用并非关键因素。从这一结果推断，sufS601通过双重解码导致移码。
相似文献
1
Suppression of a -1 frameshift mutation by a recessive tRNA suppressor which causes doublet decoding.一种隐性tRNA抑制子对-1移码突变的抑制，该抑制子导致双联体解码。
J Bacteriol. 1989 Jul;171(7):3824-30. doi: 10.1128/jb.171.7.3824-3830.1989.
2
Genetic characterization of frameshift suppressors with new decoding properties.具有新解码特性的移码抑制子的遗传特征分析。
J Bacteriol. 1989 Feb;171(2):1028-34. doi: 10.1128/jb.171.2.1028-1034.1989.
3
Competition between frameshifting, termination and suppression at the frameshift site in the Escherichia coli release factor-2 mRNA.大肠杆菌释放因子-2 mRNA移码位点处移码、终止和抑制之间的竞争。
Nucleic Acids Res. 1993 Nov 11;21(22):5074-8. doi: 10.1093/nar/21.22.5074.
4
Doublet translocation at GGA is mediated directly by mutant tRNA(2Gly).GGA处的双峰易位直接由突变型tRNA（2Gly）介导。
J Bacteriol. 1992 Jun;174(12):4179-82. doi: 10.1128/jb.174.12.4179-4182.1992.
5
tRNA imbalance promotes -1 frameshifting via near-cognate decoding.转运RNA失衡通过近同源解码促进-1移码。
J Mol Biol. 1998 Jun 19;279(4):727-36. doi: 10.1006/jmbi.1998.1832.
6
Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.上下文效应：抑制性tRNA对UAG密码子的翻译受到信使RNA中UAG之后序列的影响。
J Mol Biol. 1983 Feb 15;164(1):73-87. doi: 10.1016/0022-2836(83)90088-8.
7
Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ.四碱基密码子ACCA、ACCU和ACCC可被移码抑制因子sufJ识别。
Cell. 1981 Aug;25(2):489-96. doi: 10.1016/0092-8674(81)90067-2.
8
Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae.酿酒酵母SUF2移码抑制基因的核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3565-9. doi: 10.1073/pnas.79.11.3565.
9
Increased ribosomal accuracy increases a programmed translational frameshift in Escherichia coli.核糖体准确性的提高会增加大肠杆菌中一种程序性翻译移码。
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2315-9. doi: 10.1073/pnas.90.6.2315.
10
Insertion (sufB) in the anticodon loop or base substitution (sufC) in the anticodon stem of tRNA(Pro)2 from Salmonella typhimurium induces suppression of frameshift mutations.鼠伤寒沙门氏菌tRNA(Pro)2反密码子环中的插入（sufB）或反密码子茎中的碱基替换（sufC）可诱导移码突变的抑制。
Nucleic Acids Res. 1992 Jul 11;20(13):3463-9. doi: 10.1093/nar/20.13.3463.
引用本文的文献
1
A gripping tale of ribosomal frameshifting: extragenic suppressors of frameshift mutations spotlight P-site realignment.一个关于核糖体移码的引人入胜的故事：移码突变的基因外抑制子突显P位点重排。
Microbiol Mol Biol Rev. 2009 Mar;73(1):178-210. doi: 10.1128/MMBR.00010-08.
2
Mutations which alter the elbow region of tRNA2Gly reduce T4 gene 60 translational bypassing efficiency.改变tRNA2Gly肘部区域的突变会降低T4基因60的翻译跳跃效率。
EMBO J. 1999 May 17;18(10):2886-96. doi: 10.1093/emboj/18.10.2886.
3
Functional tRNAs with altered 3' ends.3' 端发生改变的功能性转运RNA。
EMBO J. 1993 Jun;12(6):2559-66. doi: 10.1002/j.1460-2075.1993.tb05911.x.
4
Genetic map of Salmonella typhimurium, edition VIII.鼠伤寒沙门氏菌遗传图谱，第八版。
Microbiol Rev. 1995 Jun;59(2):241-303. doi: 10.1128/mr.59.2.241-303.1995.
5
Glycine tRNA mutants with normal anticodon loop size cause -1 frameshifting.具有正常反密码子环大小的甘氨酸tRNA突变体导致-1移码。
Proc Natl Acad Sci U S A. 1989 Oct;86(20):7979-83. doi: 10.1073/pnas.86.20.7979.
6
Overproduction of release factor reduces spontaneous frameshifting and frameshift suppression by mutant elongation factor Tu.释放因子的过量产生会减少自发移码以及突变延伸因子Tu介导的移码抑制。
J Bacteriol. 1990 Dec;172(12):6721-6. doi: 10.1128/jb.172.12.6721-6726.1990.
7
A minor arginine tRNA mutant limits translation preferentially of a protein dependent on the cognate codon.一个微小的精氨酸转运RNA突变体优先限制依赖同源密码子的蛋白质的翻译。
J Bacteriol. 1990 May;172(5):2504-10. doi: 10.1128/jb.172.5.2504-2510.1990.
8
Doublet translocation at GGA is mediated directly by mutant tRNA(2Gly).GGA处的双峰易位直接由突变型tRNA（2Gly）介导。
J Bacteriol. 1992 Jun;174(12):4179-82. doi: 10.1128/jb.174.12.4179-4182.1992.
本文引用的文献
1
The nucleotide sequence of the first externally suppressible--1 frameshift mutant, and of some nearby leaky frameshift mutants.首个可外部抑制的 -1 移码突变体以及一些附近的渗漏移码突变体的核苷酸序列。
EMBO J. 1983;2(8):1345-50. doi: 10.1002/j.1460-2075.1983.tb01590.x.
2
Dual function transcripts specifying tRNA and mRNA.指定tRNA和mRNA的双功能转录本。
Nature. 1981 Dec 3;294(5840):422-7. doi: 10.1038/294422a0.
3
The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.大肠杆菌tufB及四个相邻tRNA结构基因的核苷酸序列。
Gene. 1980 Dec;12(1-2):33-9. doi: 10.1016/0378-1119(80)90013-x.
4
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.噬菌体T7 DNA的完整核苷酸序列及T7遗传元件的定位
J Mol Biol. 1983 Jun 5;166(4):477-535. doi: 10.1016/s0022-2836(83)80282-4.
5
RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.大肠杆菌中RNA聚合酶利福平抗性突变：序列变化与显性
Mol Gen Genet. 1983;190(2):344-8. doi: 10.1007/BF00330662.
6
Codon recognition by glycine transfer RNAs of Escherichia coli in vivo.大肠杆菌甘氨酸转运RNA在体内对密码子的识别
J Mol Biol. 1980 Apr 25;138(4):833-44. doi: 10.1016/0022-2836(80)90067-4.
7
New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.用于转座子诱变及通过转座构建lacZ操纵子融合体的新型Tn10衍生物。
Gene. 1984 Dec;32(3):369-79. doi: 10.1016/0378-1119(84)90012-x.
8
Suppressor sufJ: a novel type of tRNA mutant that induces translational frameshifting.抑制因子sufJ：一种诱导翻译移码的新型tRNA突变体。
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6105-9. doi: 10.1073/pnas.81.19.6105.
9
Classification of aminotransferase (C gene) mutants in the histidine operon.组氨酸操纵子中氨基转移酶（C基因）突变体的分类
J Mol Biol. 1966 Nov 14;21(2):335-55. doi: 10.1016/0022-2836(66)90103-3.
10
Histidinol dehydrogenase (his D) mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的组氨醇脱氢酶（his D）突变体。
J Bacteriol. 1971 May;106(2):421-31. doi: 10.1128/jb.106.2.421-431.1971.
Zotero 插件