Division of Biological Sciences, University of Montana, Missoula, Montana, USA.
Department of Biology, University of Colorado, Colorado Springs, Colorado, USA.
J Bacteriol. 2018 Jun 11;200(13). doi: 10.1128/JB.00035-18. Print 2018 Jul 1.
The rRNA genes of () are unusually organized; the spirochete has a single 16S rRNA gene that is more than 3 kb from a tandem pair of 23S-5S rRNA operons. We generated an null mutant in that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. Here, we demonstrate that endoribonuclease III (RNase III) is, as expected, involved in processing the 23S rRNA in The 5' and 3' ends of the three rRNAs were determined in the wild type and mutants; the results suggest that RNase III in is required for the full maturation of the 23S rRNA but not for the 5S rRNA nor, curiously, for the 16S rRNA. Lyme disease, the most common tick-borne zoonosis in the Northern Hemisphere, is caused by the bacterium () , a member of the deeply branching spirochete phylum. carries a limited suite of ribonucleases, enzymes that cleave RNA during processing and degradation. Several ribonucleases, including RNase III, are involved in the production of ribosomes, which catalyze translation and are a major target of antibiotics. This is the first study to dissect the role of an RNase in any spirochete. We demonstrate that an RNase III mutant is viable but has altered processing of rRNA.
（）的 rRNA 基因组织异常；该螺旋体只有一个 16S rRNA 基因，距离串联的 23S-5S rRNA 操纵子对超过 3kb。我们在 中生成了一个 缺失突变体，该突变体表现出多种表型，包括生长速度降低和细胞长度增加。在这里，我们证明内切核酸酶 III（RNase III）如预期的那样，参与 中 23S rRNA 的加工。在野生型和 突变体中确定了三个 rRNA 的 5'和 3'末端；结果表明， 在 中，RNase III 是 23S rRNA 完全成熟所必需的，但不是 5S rRNA 所必需的，而且奇怪的是，也不是 16S rRNA 所必需的。莱姆病是北半球最常见的蜱传动物病，由细菌 引起，它是深分支螺旋体门的成员。 携带有限的核糖核酸酶套件，这些酶在加工和降解过程中切割 RNA。几种核糖核酸酶，包括 RNase III，参与核糖体的生成，核糖体催化翻译，是抗生素的主要靶点。这是第一项研究核糖核酸酶在任何螺旋体中的作用的研究。我们证明，RNase III 突变体是可行的，但 rRNA 的加工发生了改变。
