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23S/5S核糖体RNA基因（rrl/rrf）在莱姆病的病原体伯氏疏螺旋体中与16S核糖体RNA基因（rrs）是分开的。

The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease.

作者信息

Fukunaga M, Yanagihara Y, Sohnaka M

机构信息

Faculty of Pharmacy and Pharmaceutical Sciences, University of Fukuyama, Hiroshima, Japan.

出版信息

J Gen Microbiol. 1992 May;138(5):871-7. doi: 10.1099/00221287-138-5-871.

DOI:10.1099/00221287-138-5-871
PMID:1379622
Abstract

DNA fragments containing the rRNA genes for Borrelia burgdorferi strain B31 were cloned in bacteriophage lambda EMBL3. A restriction map of the fragments was constructed and the organization of the rRNA genes was determined by Southern hybridization. One genomic DNA fragment contained a single copy of the rrs sequence and another cloned fragment contained both rrl and rrf sequences. The results revealed that the rrs gene is located separately from the set of rrl/rrf genes, suggesting that these rRNA genes are expressed independently in B. burgdorferi.

摘要

含有伯氏疏螺旋体菌株B31的rRNA基因的DNA片段被克隆到噬菌体λEMBL3中。构建了这些片段的限制性图谱，并通过Southern杂交确定了rRNA基因的组织方式。一个基因组DNA片段包含rrs序列的单拷贝，另一个克隆片段包含rrl和rrf序列。结果表明，rrs基因与rrl/rrf基因集是分开定位的，这表明这些rRNA基因在伯氏疏螺旋体中是独立表达的。

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1
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2
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