Department of Biochemistry, St. Thomas College, Pala, Kottayam, Kerala, India.
Inorganic and Physical Chemistry Department, Indian Institute of Science, Bangalore, Karnataka, India.
Inflammopharmacology. 2018 Oct;26(5):1273-1282. doi: 10.1007/s10787-018-0474-0. Epub 2018 Apr 9.
Oxidised low-density lipoprotein (ox-LDL) is a pro-atherogenic molecule, which induces inflammatory response and contributes to the pathogenesis of vascular dysfunction to atherosclerosis. The aim of the present study was to explore the anti-inflammatory effect of a novel bioavailable formulation of curcumin as 'curcumagalactomannosides' (CGM) against ox-LDL-induced inflammatory responses in human peripheral blood mononuclear cells (hPBMCs). Curcumagalactomannosides was made from natural curcumin using the soluble dietary fibre (galactomannans) derived from fenugreek seeds (Trigonella foenumgracum) and the hPBMCs were isolated from healthy human volunteers. The cells were cultured in collagen-coated plates at 37 °C and grouped as Group I (Control), Group II (ox-LDL treated) and Group III (ox-LDL + CGM treated). Further analysis of inflammatory markers, reactive oxygen species and mRNA expression levels indicated significantly increased expressions of iNOS, TNF-α, IL-6 and VCAM-1 in ox-LDL-treated group along with the nuclear translocation of NF-κB. Other inflammatory markers such as LOX, PGE2, total COX and lipid peroxidation level were also found to be significantly (p < 0.05) increased upon ox-LDL treatment. The treatment with CGM on the other hand was found to down-regulate and reverse the ox-LDL-induced alterations indicating its potential anti-inflammatory effect on hPBMCs via. NF-κB signalling pathway.
氧化型低密度脂蛋白（ox-LDL）是一种促动脉粥样硬化的分子，它可诱导炎症反应，并促进血管功能障碍向动脉粥样硬化的发展。本研究旨在探讨新型姜黄素可生物利用制剂——姜黄素半乳甘露寡糖（CGM）对 ox-LDL 诱导的人外周血单个核细胞（hPBMC）炎症反应的抗炎作用。CGM 是从天然姜黄素中提取出来的，使用的是来自葫芦巴种子（Trigonella foenumgracum）的可溶性膳食纤维（半乳甘露聚糖）。hPBMC 从健康的人类志愿者中分离出来。细胞在涂有胶原蛋白的平板中于 37°C 下培养，并分为三组：I 组（对照组）、II 组（ox-LDL 处理组）和 III 组（ox-LDL+CGM 处理组）。对炎症标志物、活性氧和 mRNA 表达水平的进一步分析表明，ox-LDL 处理组的 iNOS、TNF-α、IL-6 和 VCAM-1 的表达显著增加，同时 NF-κB 发生核转位。其他炎症标志物，如 LOX、PGE2、总 COX 和脂质过氧化水平也发现明显增加（p<0.05）。另一方面，CGM 的处理被发现可下调和逆转 ox-LDL 诱导的改变，表明其通过 NF-κB 信号通路对 hPBMC 具有潜在的抗炎作用。
