Cardiovascular Medical Research Center and Department of Diagnostics, College of Oriental Medicine, Dongguk University, Sukjang-Dong 707, Gyeong-Ju 780-714, Republic of Korea.
J Enzyme Inhib Med Chem. 2010 Oct;25(5):720-9. doi: 10.3109/14756360903555274.
In this study, the anti-oxidative activities of 70% ethanol extract from Curcuma aromatica Salisb. (CAS) and curcumin (CUR) were studied. The CAS extracts and CUR were both found to have a potent scavenging activity against the reactive species tested, as well as an inhibitory effect on LDL oxidation. Cultured human umbilical vein endothelial cells (HUVECs) were stimulated with tumour necrosis factor alpha (TNFalpha), expression of intracellular reactive oxygen species (ROS), nitric oxide (NO), endothelial nitric oxide synthase (eNOS), lectin-like oxidised LDL receptor-1 (LOX-1), adhesion molecules, inhibitory kappa Balpha (IkappaBalpha) and nuclear factor kappa B (NFkappaB) were measured. In HUVECs stimulated with TNFalpha, CUR significantly suppressed expression of the intracellular ROS, LOX-1 and adhesion molecules, degradation of IkappaBalpha and translocation of NFkappaB, while inducing production of NO by phosphorylation of eNOS (p <0.05). In conclusion, CAS and CUR may modulate lipoprotein composition and attenuate oxidative stress by elevated antioxidant processes.
本研究旨在探讨姜黄（Curcuma aromatica Salisb.）70%乙醇提取物（CAS）和姜黄素（CUR）的抗氧化活性。结果发现，CAS 提取物和 CUR 均对所测试的活性物质具有强大的清除活性，并且对 LDL 氧化具有抑制作用。用肿瘤坏死因子-α（TNFα）刺激人脐静脉内皮细胞（HUVEC），测量细胞内活性氧（ROS）、一氧化氮（NO）、内皮型一氧化氮合酶（eNOS）、凝集素样氧化型 LDL 受体-1（LOX-1）、黏附分子、抑制κB 亚基（IkappaBalpha）和核因子κB（NFkappaB）的表达。结果显示，在 TNFα 刺激的 HUVEC 中，CUR 可显著抑制细胞内 ROS、LOX-1 和黏附分子的表达，抑制 IkappaBalpha 的降解和 NFkappaB 的易位，同时通过 eNOS 的磷酸化诱导 NO 的产生（p＜0.05）。综上所述，CAS 和 CUR 可能通过增强抗氧化过程来调节脂蛋白组成并减轻氧化应激。
