Gao Xumei, Stoecklin Celine, Zhang Yue, Weng Zhe, De Mets Richard, Grenci Gianluca, Viasnoff Virgile
Mechanobiology Institute, National University of Singapore, Singapore, Singapore.
Centre National Pour la Recherche Scientifique, UMI3639, Singapore, Singapore.
Methods Mol Biol. 2018;1771:55-66. doi: 10.1007/978-1-4939-7792-5_5.
We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments has been already established and is referenced.
我们在此展示一种创建微腔阵列的方法，这些微腔能够在其底部、侧面和顶部用不同蛋白质进行差异化包被。这些微腔的大小范围从单细胞到多细胞聚集体。我们提供了详细的方案，可使用不同材料和不同包被蛋白质进行一些变化来创建此类阵列。将此类微腔用作真正的人工微环境来模拟细胞微环境的用途已经确立并被引用。
