Alemdar Neslihan
Department of Chemical Engineering, Marmara University, Istanbul, Turkey.
Methods Mol Biol. 2018;1771:241-250. doi: 10.1007/978-1-4939-7792-5_19.
Providing sufficient amount of oxygen to the cells is a critical issue since the lack of adequate oxygen leads to cell death and tissue necrosis. Therefore, there is a vital need to design and fabricate oxygen-generating biomaterials to mitigate hypoxia-induced cell death in engineered tissues. Here, we report the fabrication of an oxygen-generating hydrogel by incorporating calcium peroxide (CPO) into the methacrylated gelatin (GelMA) structure using photocrosslinking process. A sustainable release of oxygen could be provided from CPO-GelMA hydrogel over a period of 5 days under hypoxic conditions (1% O).
为细胞提供足够的氧气是一个关键问题，因为缺乏足够的氧气会导致细胞死亡和组织坏死。因此，迫切需要设计和制造产氧生物材料，以减轻工程组织中缺氧诱导的细胞死亡。在此，我们报告了通过光交联过程将过氧化钙（CPO）掺入甲基丙烯酸化明胶（GelMA）结构中来制造产氧水凝胶。在缺氧条件（1% O）下，CPO-GelMA水凝胶可在5天内持续释放氧气。
