LIMAV - Interdisciplinary Laboratory for Advanced Materials, BioMatLab, UFPI - Federal University of Piauí, Teresina, PI, 64049-550, Brazil.
Division of Engineering in Medicine, Department of Medicine, Brigham & Women´s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA; Harvard-MIT Division of Health Science and Technology, MIT - Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Colloids Surf B Biointerfaces. 2020 Oct;194:111192. doi: 10.1016/j.colsurfb.2020.111192. Epub 2020 Jun 13.
Cartilage is one of our body's tissues which are not repaired automatically by itself. Problems associated with cartilage are very common worldwide and are considered the leading cause of pain and disability. Smart biomaterial or "Four dimensional" (4D) biomaterials has started emerging as a suitable candidate, which are principally three dimensional (3D) materials that change their morphology or generate a response measured at space and time to physiologic stimuli. In this context, the release of oxygen through hydrogels in contact with water is considered as 4D biomaterials. The objective of this study is to develop strategies to release oxygen in a sustainable and prolonged manner through hydrogels systems to promote chondrocytes survival in oxygen-free environment. The 4D biomaterials are engineered from gelatin methacryloyl (GelMA) loaded with calcium peroxide (CPO), which have the ability to generate oxygen in a controlled and sustained manner for up to 6 days. The incorporation of CPO into the hydrogel system provided materials with enhanced mechanical and porosity properties. Furthermore, the hydrogels promoted chondrocyte survival and reduced cell death under oxygen-free conditions.
软骨是我们身体的组织之一，它不会自动自我修复。与软骨相关的问题在全球范围内非常普遍，被认为是疼痛和残疾的主要原因。智能生物材料或“四维”（4D）生物材料开始崭露头角，它们主要是三维（3D）材料，能够改变其形态或在空间和时间上对生理刺激产生反应。在这种情况下，通过与水接触的水凝胶释放氧气被认为是 4D 生物材料。本研究的目的是开发通过水凝胶系统以可持续和延长的方式释放氧气的策略，以促进软骨细胞在无氧环境中的存活。4D 生物材料是由明胶甲基丙烯酰（GelMA）负载过氧钙（CPO）制成的，具有在长达 6 天的时间内以受控和持续的方式产生氧气的能力。将 CPO 掺入水凝胶系统为材料提供了增强的机械和多孔性特性。此外，水凝胶在无氧条件下促进了软骨细胞的存活并减少了细胞死亡。
