Behmanesh Mohammad Amin, Najafzadehvarzi Hosein, Poormoosavi Seyedeh Mahsa
Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran.
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Cell J. 2018 Jul;20(2):278-283. doi: 10.22074/cellj.2018.5256. Epub 2018 Mar 18.
Bisphenol A (BPA), an endocrine-disrupting chemical, has been considered as a possible risk factor for fertility because it induces testicular toxicity. Thus, we sought to analyze the effect of Aloe vera as plant with antioxidant properties on tissues and oxidative stress parameters in male rats.
In this experimental study, 50 adult male Wistar rats (200 ± 20 g) have been used in this 56 day study. Animals were completely randomized and divided into five groups: A (control), A (vehicle control), A (Aloe vera gel 300 mg/kg), B (BPA 20 μg/kg bw) and B (Aloe vera gel+ BPA). At the end of the study, the rats were anesthetized and 2 ml blood samples were obtained for evaluation of oxidative stress markers. Also, both testes were collected for histological examinations.
BPA significantly decreased (P<0.05) body and testis weights. Seminiferous tubule diameter (STD) and height of seminiferous epithelium (HSE), were significantly decreased (P<0.05) in the groups receiving BPA as compared to the control. There was also a reduction in the quantity of spermatocyte and spermatids. Moreover, malondialdehyde (MDA) increased and thiol protein (G-SH) decreased. But, co-administration of Aloe vera with BPA accelerated the total antioxidant capacity and testicular tissue structure healing.
According to our findings, Aloe vera gel extract can overcome the damaging effects of BPA on the reproductive system of rats and protects rats' testes against BPA-induced toxicity.
双酚A（BPA）是一种内分泌干扰化学物质，因其会诱发睾丸毒性，被认为是生育能力的一个潜在风险因素。因此，我们试图分析具有抗氧化特性的植物芦荟对雄性大鼠组织和氧化应激参数的影响。
在这项实验研究中，50只成年雄性Wistar大鼠（体重200±20克）被用于这项为期56天的研究。动物被完全随机分组，分为五组：A组（对照组）、A组（溶剂对照组）、A组（芦荟凝胶300毫克/千克）、B组（BPA 20微克/千克体重）和B组（芦荟凝胶+BPA）。在研究结束时，大鼠被麻醉，采集2毫升血液样本用于评估氧化应激标志物。同时，收集双侧睾丸进行组织学检查。
BPA显著降低了（P<0.05）体重和睾丸重量。与对照组相比，接受BPA的组中，生精小管直径（STD）和生精上皮高度（HSE）显著降低（P<0.05）。精母细胞和精子细胞数量也有所减少。此外，丙二醛（MDA）增加，硫醇蛋白（G-SH）减少。但是，芦荟与BPA联合使用可提高总抗氧化能力并促进睾丸组织结构的恢复。
根据我们的研究结果，芦荟凝胶提取物可以克服BPA对大鼠生殖系统的损害作用，并保护大鼠睾丸免受BPA诱导的毒性。
