Abdelhafidh Khazri, Badreddine Sellami, Mezni Amine, Mouhamed Dellali, Wiem Saidani, Imen Bouzidi, David Sheehan, Mahmoudi Ezzeddine, Hamouda Beyrem
a Faculté des Sciences de Bizerte , Laboratoire de Biosurveillance de l'Environnement (LBE), Unité d'Ecotoxicologie et d'Ecologie Côtière (GREEC) , Zarzouna-Bizerte , Tunisia.
b National Institute of Marine Sciences and Technologies , Tabarka , Tunisia.
Biomarkers. 2018 Sep;23(6):580-588. doi: 10.1080/1354750X.2018.1463565. Epub 2018 Apr 19.
Nanoparticles may cause adverse environmental effects but there is limited information on their interactions with marine organisms.
Our aim was to examine the effects of triangular gold nanoparticles (Tr-Au NPs) on the clam, Ruditapes decussatus.
Clams were exposed to Tr-Au1 = 5 µg/L and Tr-Au2 = 10 µg/L for 2 and 7 days. Effects on shell structure were investigated. Superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST) activities, protein carbonyl levels and malondialdehyde content were used to assess biochemical status.
Transmission electron microscopy (TEM) and electron dispersive X-ray microanalysis (EDX) showed that Tr-Au NPs modified shell structure and morphology. Tr-Au NPs size increased forming aggregate particles. Tr-Au NPs increased SOD, CAT and GST activities in gill and digestive gland in a concentration- and time-dependent manner indicating defence against oxidative stress. Enhanced lipid peroxidation and protein carbonyl levels confirmed oxidative stress.
Tr-Au NPs cause oxidative stress and affect shell structure of clams. These findings may have relevance to other marine species.
纳米颗粒可能会对环境产生不利影响，但关于它们与海洋生物相互作用的信息有限。
我们的目的是研究三角形金纳米颗粒（Tr-Au NPs）对蛤仔（Ruditapes decussatus）的影响。
将蛤仔暴露于Tr-Au1 = 5 μg/L和Tr-Au2 = 10 μg/L的环境中2天和7天。研究对贝壳结构的影响。使用超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽转移酶（GST）活性、蛋白质羰基水平和丙二醛含量来评估生化状态。
透射电子显微镜（TEM）和能量色散X射线微分析（EDX）表明，Tr-Au NPs改变了贝壳的结构和形态。Tr-Au NPs尺寸增大并形成聚集颗粒。Tr-Au NPs以浓度和时间依赖性方式增加了鳃和消化腺中SOD、CAT和GST的活性，表明对氧化应激有防御作用。脂质过氧化和蛋白质羰基水平的升高证实了氧化应激。
Tr-Au NPs引起氧化应激并影响蛤仔的贝壳结构。这些发现可能与其他海洋物种有关。
