Tomar R S, Blakley B R, De Coteau W E
Department of Veterinary Physiological Sciences, University of Saskatchewan, Saskatoon, Canada.
Toxicol Lett. 1988 Feb;40(2):109-17. doi: 10.1016/0378-4274(88)90151-8.
The effects of T-2 toxin on the in vitro mitogen responses and the antibody-producing ability of human peripheral blood lymphocytes were evaluated. T-2 toxin inhibited the mitogen response to concanavalin A (ConA) at a lower concentration (1.6 ng/ml) as compared to phytohemagglutinin (2.4 ng/ml) and pokeweed mitogen (2.4 ng/ml). Maximum inhibition was observed when the toxin was present during the first 8 h; however, the cultures were not refractory to inhibition until 48 h after culture initiation. The antibody-producing ability was inhibited by T-2 toxin concentrations of greater than or equal to 3.2 ng/ml. T-2 toxin did not induce or interfere with the generation of suppressor cells by ConA. The results of this study indicate that various lymphocyte subpopulations have different susceptibilities to T-2 toxin. The activation process associated with lymphocyte proliferation appears to be one of the most sensitive time periods.
评估了T-2毒素对人外周血淋巴细胞体外有丝分裂原反应及抗体产生能力的影响。与植物血凝素（2.4 ng/ml）和商陆有丝分裂原（2.4 ng/ml）相比，T-2毒素在较低浓度（1.6 ng/ml）时就能抑制对刀豆球蛋白A（ConA）的有丝分裂原反应。当毒素在最初8小时内存在时观察到最大抑制作用；然而，直到培养开始后48小时，培养物才对抑制作用产生抗性。当T-2毒素浓度大于或等于3.2 ng/ml时，抗体产生能力受到抑制。T-2毒素不会诱导或干扰ConA诱导的抑制性细胞的产生。本研究结果表明，不同的淋巴细胞亚群对T-2毒素有不同的敏感性。与淋巴细胞增殖相关的激活过程似乎是最敏感的时期之一。
