Laboratory of Pharmacology, Neurobiology and Behavior URAC-37, Cadi Ayyad University, Marrakech, Morocco.
Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences, Cadi Ayyad University, Marrakech, Morocco.
Neurotoxicol Teratol. 2018 May-Jun;67:44-49. doi: 10.1016/j.ntt.2018.04.002. Epub 2018 Apr 7.
Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and its subsequent effects on the neurobehavioral functions in mice following acute, subchronic and chronic exposure to 250 or 500 mg/kg/day. The effect of these acute and repeated treatments was assessed at the behavioral level using the open field, the elevated plus maze, the tail suspension and splash tests. Then, mice were sacrificed and the intestinal samples were collected for GM analysis. Subchronic and chronic exposure to GBH induced an increase of anxiety and depression-like behaviors. In addition, GBH significantly altered the GM composition in terms of relative abundance and phylogenic diversity of the key microbes. Indeed, it decreased more specifically, Corynebacterium, Firmicutes, Bacteroidetes and Lactobacillus in treated mice. These data reinforce the essential link between GM and GBH toxicity in mice and suggest that observed intestinal dysbiosis could increase the prevalence of neurobehavioral alterations.
最近，许多研究表明肠道微生物群（GM）的改变与行为变化之间存在着深刻的关系。草甘膦基除草剂（GBH）已被证明会导致行为障碍，并且有可能通过改变 GM 来介导这些影响。在这项研究中，我们研究了 GBH 对 GM 的毒性作用，以及在急性、亚慢性和慢性暴露于 250 或 500mg/kg/天后，GM 对小鼠神经行为功能的后续影响。使用开阔场、高架十字迷宫、悬尾和溅水测试在行为水平上评估这些急性和重复处理的效果。然后，处死小鼠并收集肠道样本进行 GM 分析。亚慢性和慢性暴露于 GBH 会导致焦虑和抑郁样行为增加。此外，GBH 还显著改变了 GM 的组成，包括关键微生物的相对丰度和系统发育多样性。实际上，它在治疗小鼠中特异性地减少了 Corynebacterium、Firmicutes、Bacteroidetes 和 Lactobacillus。这些数据强化了 GM 和 GBH 毒性在小鼠中的重要联系，并表明观察到的肠道菌群失调可能会增加神经行为改变的流行率。
