NeuroPoly Lab, Institute of Biomedical Engineering, Polytechnique Montreal, Montreal, QC, Canada; Functional Neuroimaging Unit, CRIUGM, Université de Montréal, Montreal, QC, Canada.
Neuroimage. 2018 Nov 15;182:169-183. doi: 10.1016/j.neuroimage.2018.04.009. Epub 2018 Apr 7.
In vivo histology using magnetic resonance imaging (MRI) is a newly emerging research field that aims to non-invasively characterize tissue microstructure. The implications of in vivo histology are many, from discovering novel biomarkers to studying human development, to providing tools for disease diagnosis and monitoring the effects of novel treatments on tissue. This review focuses on quantitative MRI (qMRI) techniques that are used to map spinal cord microstructure. Opening with a rationale for non-invasive imaging of the spinal cord, this article continues with a brief overview of the existing MRI techniques for axon and myelin imaging, followed by the specific challenges and potential solutions for acquiring and processing such data. The final part of this review focuses on histological validation, with suggested tissue preparation, acquisition and processing protocols for large-scale microscopy.
体内组织学使用磁共振成像（MRI）是一个新兴的研究领域，旨在非侵入性地描述组织的微观结构。体内组织学的应用非常广泛，从发现新的生物标志物到研究人类发育，再到为疾病诊断提供工具以及监测新疗法对组织的影响。这篇综述重点介绍了用于脊髓微观结构成像的定量磁共振成像（qMRI）技术。本文首先介绍了对脊髓进行非侵入性成像的基本原理，接着简要概述了现有的用于轴突和髓鞘成像的 MRI 技术，然后介绍了获取和处理此类数据所面临的具体挑战和潜在解决方案。这篇综述的最后一部分重点介绍了组织学验证，提出了用于大规模显微镜检查的组织准备、采集和处理方案。
