Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Huaihai Institute of Technology, Lianyungang, 222005, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Fish Shellfish Immunol. 2019 Jan;84:962-969. doi: 10.1016/j.fsi.2018.10.030. Epub 2018 Nov 3.
STAT plays important roles in innate immunity during JAK/STAT signaling pathway, and STAT5 is particularly focused due to the existence of duplicated forms in fish and mammal. In Chinese tongue sole, stat5bl was suggested to be a candidate related to Vibrio harveyi resistance based on previous QTL screening. In this study, the full length of stat5bl cDNA was cloned and its expression patterns were analyzed. stat5bl was predominantly expressed in immune tissues, where the highest level was observed in liver, followed by skin and gill. Time course expression patterns were examined in six tissues (liver, skin, gill, kidney, intestine, spleen) after V. harveyi infection. stat5bl could be up-regulated by V. harveyi infection in all tissues except liver, despite the timepoints of peak were different. In contrast, stat5bl was significantly downregulated in liver. To elucidate the role of stat5bl in liver, in vitro RNAi were performed using primary liver cell culture. Knockdown of stat5bl could regulate the expression of genes closely related to JAK/STAT pathway. This study would enlarge our understanding of stat5bl in fish immunity.
STAT 在 JAK/STAT 信号通路中的先天免疫中发挥重要作用，由于鱼类和哺乳动物中存在重复形式，STAT5 尤为受到关注。在牙鲆中，根据之前的 QTL 筛选，stat5bl 被认为是与哈维氏弧菌抗性相关的候选基因。在这项研究中，克隆了 stat5bl cDNA 的全长，并分析了其表达模式。stat5bl 在免疫组织中表达丰度较高，在肝脏中表达水平最高，其次是皮肤和鳃。在哈维氏弧菌感染后，检测了六个组织（肝脏、皮肤、鳃、肾脏、肠道、脾脏）中的时间过程表达模式。stat5bl 在除肝脏以外的所有组织中都可以被哈维氏弧菌感染上调，尽管峰值时间点不同。相比之下，stat5bl 在肝脏中显著下调。为了阐明 stat5bl 在肝脏中的作用，我们在原代肝实质细胞培养中进行了体外 RNAi。stat5bl 的敲低可以调节与 JAK/STAT 通路密切相关的基因的表达。这项研究将扩大我们对鱼类免疫中 stat5bl 的理解。
