长链非编码 RNA ANRIL 通过靶向 miR-125a 在口腔癌发生中的作用。
The role of long non-coding RNA ANRIL in the carcinogenesis of oral cancer by targeting miR-125a.
机构信息
Department of Stomatology, Ningbo Yinzhou People's Hospital (Yinzhou Hospital Affiliated to Medical School of Ningbo University), Ningbo 315040, Zhejiang, PR China.
Department of Stomatology Technology, Ningbo Colloge Of Health Sciences, Ningbo 315040, Zhejiang, PR China.
出版信息
Biomed Pharmacother. 2018 Jul;103:38-45. doi: 10.1016/j.biopha.2018.01.105. Epub 2018 Apr 24.
Recently, increasing evidence has indicated that lncRNAs may play a critical role in the progression of oral cancer (OC). However, whether lncRNA-ANRIL is involved in the tumorigenesis of OC remains undetermined. In the present study, ANRIL showed significantly higher, while miR-125a showed lower, expression in OC tissues and sera than in normal controls. MTT, colony formation, flow cytometry analysis, wound-healing, transwell and mice xenograft model assays were used to detect the proliferation, migration, and invasion of ARNIL-overexpressing HB56 cells and ARNIL-knockdown CAL27 cells. The results showed that cell proliferation, migration, and invasion were significantly increased by ARNIL overexpression and decreased by ARNIL silencing in oral cancer cells. Furthermore, we found a negative correlation between ARNIL and miR-125a, and ARNIL acts as a miRNA-sponge by directly interacting with miR-125a.
最近，越来越多的证据表明 lncRNAs 可能在口腔癌（OC）的进展中发挥关键作用。然而，lncRNA-ANRIL 是否参与 OC 的肿瘤发生仍未确定。在本研究中，与正常对照组相比，OC 组织和血清中的 ANRIL 表达明显升高，而 miR-125a 表达则明显降低。MTT、集落形成、流式细胞术分析、划痕愈合、Transwell 和小鼠异种移植模型检测显示，过表达 ANRIL 的 HB56 细胞和敲低 ANRIL 的 CAL27 细胞的增殖、迁移和侵袭能力显著增强。结果表明，在口腔癌细胞中，ANRIL 的过表达显著促进细胞增殖、迁移和侵袭，而 ANRIL 的沉默则降低了细胞的这些能力。此外，我们发现 ANRIL 与 miR-125a 之间存在负相关，并且 ANRIL 通过与 miR-125a 直接相互作用充当 miRNA 海绵。
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