Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
J Pharm Pharmacol. 2018 Jul;70(7):865-873. doi: 10.1111/jphp.12915. Epub 2018 Apr 10.
This study was aimed to investigate the in vitro permeation potential of hydrogel formulations containing the isoflavones formononetin and biochanin A and cyclodextrins in different combinations.
The permeation assay was performed using porcine skin discs on Franz diffusion cells model. The isoflavone contents of the formulations were quantified in the different layers of the skin using a validated HPLC-PDA method.
The isoflavones individually incorporated into the formulations showed high permeation potential, especially formononetin, after the incorporation of hydroxypropyl-β-cyclodextrin that enhanced its permeation in the epidermis and dermis. Biochanin A showed 2.7 times of permeation capacity in the epidermis and dermis mainly after incorporation of cyclodextrins in the formulations. Formononetin showed reduction in its permeation when incorporated in the formulations together to biochanin A, showing the absence of synergism.
Our results indicated a noticeable skin permeation promoting effect of HPβCD in formononetin formulation. Furthermore, formononetin and biochanin A can permeate the skin being mostly retained in the epidermis and dermis, revealing its potential use in cosmetic preparations intended to prevent skin aging.
本研究旨在考察不同组合的环糊精中包含染料木黄酮和大豆苷元的水凝胶制剂的体外渗透潜力。
Franz 扩散池模型中使用猪皮圆盘进行渗透测定。采用经验证的 HPLC-PDA 方法对制剂的不同层中的异黄酮含量进行定量。
异黄酮单独掺入制剂中表现出很高的渗透潜力，尤其是羟丙基-β-环糊精掺入后，其在表皮和真皮中的渗透增强。大豆苷元在制剂中掺入环糊精后，在表皮和真皮中的渗透能力增加了 2.7 倍。当染料木黄酮与大豆苷元一起掺入制剂中时，其渗透能力降低，表明不存在协同作用。
我们的结果表明 HPβCD 在染料木黄酮制剂中具有显著的皮肤渗透促进作用。此外，染料木黄酮和大豆苷元可以渗透皮肤，主要保留在表皮和真皮中，这表明其在预防皮肤老化的化妆品制剂中有潜在的应用。
