Chung R S, Bruch D, Dearlove J
Department of Surgery, Veterans Administration Medical Center, Syracuse, New York.
Am Surg. 1988 Feb;54(2):116-20.
A technique of endoscopic measurement of gastric mucosal blood flow using laser Doppler velocimetry was described. The technique was validated in the laboratory by performing endoscopic measurement of gastric mucosal blood flow in the dog simultaneously with application of a second probe at open operation, utilizing the same laser Doppler flowmeter. Probe pressure causing minor dimpling (less than 20 gm/cm2) was found to cause insignificant alteration of mucosal blood flow. Criteria were developed to aid separation of artifacts introduced by probe motion. A conversion factor was established for converting the readout of the instrument (Hertz x 10(2] into ml/100g/min by synchronously measuring mucosal blood flow by hydrogen gas clearance technique and laser Doppler velocimetry in the gastric and duodenal mucosa. In patients with portal hypertension gastric mucosal blood flow was determined before and after sclerotherapy, and compared to the gastric mucosal blood flow in subjects without portal hypertension. Gastric mucosal blood flow was elevated in portal hypertensives, but sclerotherapy did not appear to cause changes in blood flow. Endoscopic mucosal blood flow measurement is non-invasive, practical and of potential value in clinical investigation of gastrointestinal pathophysiology.
描述了一种使用激光多普勒测速仪进行内镜测量胃黏膜血流的技术。该技术在实验室中通过在开放手术时使用同一激光多普勒流量计，同时在狗身上进行内镜测量胃黏膜血流并应用第二个探头进行了验证。发现引起轻微压痕（小于20克/平方厘米）的探头压力对黏膜血流的改变不显著。制定了有助于分离由探头移动引入的伪像的标准。通过同步测量胃和十二指肠黏膜中的氢气清除技术和激光多普勒测速仪的黏膜血流，建立了一个将仪器读数（赫兹×10²）转换为毫升/100克/分钟的转换因子。在门静脉高压患者中，在硬化治疗前后测定胃黏膜血流，并与无门静脉高压受试者的胃黏膜血流进行比较。门静脉高压患者的胃黏膜血流升高，但硬化治疗似乎并未引起血流变化。内镜黏膜血流测量在胃肠道病理生理学的临床研究中是非侵入性的、实用的且具有潜在价值。
