Escobedo-Guerra Marcos R, Katoku-Herrera Mitzuko, Lopez-Hurtado Marcela, Villagrana-Zesati Jesus Roberto, de Haro-Cruz María de J, Guerra-Infante Fernando M
Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico; Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, CDMX, Mexico.
Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico.
Enferm Infecc Microbiol Clin (Engl Ed). 2019 Feb;37(2):93-99. doi: 10.1016/j.eimc.2018.02.008. Epub 2018 Apr 7.
Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico.
Swedish-nvCT was searched in 69C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH).
The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters.
Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.
沙眼衣原体是全球性传播感染的主要病原体之一。2006年，报告了一种瑞典沙眼衣原体变体（瑞典-nvCT），其质粒中有377bp的缺失。在拉丁美洲，尚未报告瑞典-nvCT感染情况。我们调查了墨西哥不孕妇女中瑞典-nvCT的存在情况。
在来自2339份宫颈标本的69份沙眼衣原体阳性样本中搜索瑞典-nvCT。我们设计了PCR引物，以鉴定ORF1中质粒的缺失以及ORF3中44bp重复序列的存在。对具有缺失的样本进行主要外膜蛋白A（ompA）基因和多态性膜蛋白（pmpH）基因分型。
在2339份宫颈分泌物的69份沙眼衣原体阳性样本中，有1份检测到缺失。ompA的核苷酸序列分析显示与瑞典-nvCT高度相似（98%），然而发现的变体属于血清型D。pmpH基因的核苷酸序列与瑞典-nvCT泌尿生殖道型中发现的变体相关，但在不同簇中。
我们的结果揭示了墨西哥患者中存在一种新的沙眼衣原体变体。根据ompA和pmpH基因的电子分析，在墨西哥发现的这种变体属于血清型D，与瑞典-nvCT（血清型E）不同。对于已发现的这些沙眼衣原体变体，尽管这种突变在发病机制中的作用尚未得到证实，但有必要进行更详细的分析。
