Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
WHO Collaborating Centre for Gonorrhoea and other STIs, Örebro University, Örebro, Sweden.
Sex Transm Infect. 2018 Mar;94(2):100-104. doi: 10.1136/sextrans-2016-052992. Epub 2017 Jul 19.
In 2006, a new variant of (nvCT) was discovered in Sweden. It has a deletion in the plasmid resulting in failed detection by the single target systems from Abbott and Roche used at that time, whereas the third system used, from Becton Dickinson (BD), detects nvCT. The proportion of nvCT was initially up to 65% in counties using Abbott/Roche systems. This study analysed the proportion of nvCT from 2007 to 2015 in four selected counties and its impact on chlamydia-associated complications.
-positive specimens collected from 2007 to 2015 were analysed by a specific PCR to identify nvCT cases. Genotyping was performed by multilocus sequence typing (MLST) and sequencing. Ectopic pregnancy and pelvic inflammatory disease records were extracted from the national registers.
In total, 5101 -positive samples were analysed. The nvCT proportion significantly decreased in the two counties using Roche systems, from 56% in 2007 to 6.5% in 2015 (p<0.001). In the two counties using BD systems, a decrease was also seen, from 19% in 2007 to 5.2% in 2015 (p<0.001). Fifteen nvCT cases from 2015 and 102 cases from 2006 to 2009 had identical MLST profiles. Counties using Roche/Abbott systems showed higher mean rates of ectopic pregnancy and pelvic inflammatory disease compared with counties using BD systems.
The nvCT proportion has decreased in all counties and converged to a low prevalence irrespective of previous rates. Genotyping showed that nvCT is clonal and genetically stable. Failing detection only marginally affected complication rates.
2006 年，在瑞典发现了一种新的 （nvCT）变体。它在质粒上发生缺失，导致当时使用的 Abbott 和罗氏的单一靶标系统无法检测到，而使用的第三个系统，即 Becton Dickinson（BD），可以检测到 nvCT。在最初使用 Abbott/Roche 系统的县，nvCT 的比例高达 65%。本研究分析了 2007 年至 2015 年在四个选定县的 nvCT 比例及其对衣原体相关并发症的影响。
对 2007 年至 2015 年收集的 -阳性标本进行特定 PCR 分析，以确定 nvCT 病例。通过多位点序列分型（MLST）和 测序进行基因分型。从国家登记册中提取异位妊娠和盆腔炎记录。
共分析了 5101 例 -阳性样本。在使用罗氏系统的两个县，nvCT 的比例从 2007 年的 56%显著下降到 2015 年的 6.5%（p<0.001）。在使用 BD 系统的两个县，比例也有所下降，从 2007 年的 19%下降到 2015 年的 5.2%（p<0.001）。2015 年的 15 例 nvCT 病例和 2006 年至 2009 年的 102 例病例具有相同的 MLST 图谱。使用罗氏/雅培系统的县异位妊娠和盆腔炎的平均发生率高于使用 BD 系统的县。
所有县的 nvCT 比例均有所下降，且不论先前的比率如何，均趋于低流行率。基因分型表明，nvCT 是克隆的，遗传稳定。未能检测到仅略微影响并发症的发生率。
