Department of Biomedicine, Göteborg University, The Sahlgrenska Academy, Box 435, 405 30 Göteborg, Sweden.
Eur J Clin Microbiol Infect Dis. 2010 May;29(5):609-11. doi: 10.1007/s10096-010-0887-1. Epub 2010 Mar 1.
The aims of this study were to assess the proportion of the new variant of Chlamydia trachomatis (nvCT) and the distribution of ompA genovars among C. trachomatis-positive patients in the Göteborg area, Sweden. Consecutive urine samples positive for C. trachomatis using BD ProbeTec ET (177 patients, 88 men and 89 women) were collected. An nvCT-specific real-time polymerase chain reaction (PCR) assay was used to investigate the nvCT prevalence. To identify the genovars, a 990-bp ompA DNA segment from 105 specimens was sequenced. Seventeen percent (30/177) of all specimens contained nvCT. Nine different genovars were identified. About 50% were of genovar E, followed by F 16%, G 11%, K 8%, and D 5%, representing about 90% of the specimens in Göteborg. The occurrence of nvCT and the dominance of genovar E in Göteborg is similar to those in other areas of Sweden. To cover about 90% of the C. trachomatis infections in Sweden, the serovars D, E, F, G, and K should be included in future vaccines based on the major outer membrane protein.
本研究旨在评估瑞典哥德堡地区新型沙眼衣原体（nvCT）的比例和 ompA 基因亚型在沙眼衣原体阳性患者中的分布。连续采集了 177 例使用 BD ProbeTec ET 检测呈沙眼衣原体阳性的尿样（88 名男性和 89 名女性）。采用 nvCT 特异性实时聚合酶链反应（PCR）检测 nvCT 的流行情况。为了鉴定基因亚型，对 105 个标本中的 990bp ompA DNA 片段进行了测序。所有标本中有 17%（30/177）含有 nvCT。共鉴定出 9 种不同的基因亚型。其中约 50%为基因型 E，其次是 F 16%、G 11%、K 8%和 D 5%，占哥德堡标本的约 90%。nvCT 的发生和基因型 E 的优势在哥德堡与瑞典其他地区相似。为了涵盖瑞典约 90%的沙眼衣原体感染，未来基于主要外膜蛋白的疫苗应包括血清型 D、E、F、G 和 K。
