Macías P, Pinto M C, Gutiérrez-Mérino C
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias Universidad de Extremadura, Badajoz, Spain.
Biochem Int. 1987 Nov;15(5):961-9.
We report in this paper the presence of fluorescence bands of tryptophan and tyrosine solutions centered above 550 nm. This long-wavelength fluorescence is of much lower intensity, (0.4-2.7)%, than the UV fluorescence of these aromatic aminoacids. The basic characteristic of these fluorescence bands are: (a) tyrosine: lambda em = 600 nm with two excitation peaks centered at 453 nm and 550 nm (b) tryptophan: lambda em = 675 nm with two excitation peaks centered at 455 and 560 nm. It has been found that irradiation of tyrosine solutions with a potent UV lamp promotes an important increase of absorption at 310 nm and above 400 nm.
我们在本文中报告了色氨酸和酪氨酸溶液在550nm以上的荧光带的存在。这种长波长荧光的强度比这些芳香族氨基酸的紫外荧光强度低得多，为（0.4 - 2.7）%。这些荧光带的基本特征是：（a）酪氨酸：发射波长λem = 600nm，有两个激发峰，中心分别位于453nm和550nm；（b）色氨酸：发射波长λem = 675nm，有两个激发峰，中心分别位于455nm和560nm。已发现用强紫外线灯照射酪氨酸溶液会促使其在310nm及400nm以上的吸收显著增加。
