Divya Devara, Bhattacharya Tarun Kumar, Gnana Prakash Manthani, Chatterjee R N, Shukla Renu, Guru Vishnu Pothana Boyina, Vinoth Amirthalingam, Dushyanth Kotha
College of Veterinary Science, PVNR Telangana Veterinary University, Rajendranagar, Hyderabad, India.
ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad, India.
Mol Biol Rep. 2018 Aug;45(4):477-495. doi: 10.1007/s11033-018-4184-x. Epub 2018 Apr 10.
A study was carried out to characterize and explore the expression profile of BMP 3 gene in control broiler and control layer chicken. The total open reading frame of BMP 3 (1389 bp) was cloned and sequenced. The control broiler and control layer chicken showed variation at nucleotide and amino acid level with reference gene (Gallus gallus, NCBI Acc. No. NM_001034819). When compared to reference gene, the control broiler showed four nucleotide differences (c.192A>G, c.519C>T, 903G>A and 960C>G), while, control layer showed variation at c.33G>C, 192A>G, 858G>A, 904G>A, 960C>G and 1257C>T making six differences in total. However, between control broiler and control layer lines, nucleotide differences was observed at c.33G>C, 519T>C, 858G>A, 903A>G, 904G>A and 1257C>T. The change at amino acid level between reference and control broiler was p.D320N and with control layer chicken, it was p.D302N and p.D320N. On the other hand, a single amino acid difference (p.D302N) was observed between the control broiler and control layer chicken lines. The phylogenetic study displayed a close relationship between broiler and layer lines and reference gene and also with other avian species resulting in a cluster formation. These cluster in turn displayed a distant link with the mammalian species. The expression profile of BMP 3 gene exhibited a variation at different stages of embryonic development and also at post embryonic period among the lines with control layer showing higher expression than that of broiler chicken. The protein was also detected in bone marrow tissue of broiler and layer lines by western blotting. It is concluded that the BMP 3 gene sequence differed at nucleotide and amino acid level among the lines and the gene expressed differentially at different periods of embryonic development and also at post hatch period.
开展了一项研究，以表征和探究BMP 3基因在对照肉鸡和对照蛋鸡中的表达谱。克隆并测序了BMP 3的总开放阅读框（1389 bp）。对照肉鸡和对照蛋鸡在核苷酸和氨基酸水平上与参考基因（原鸡，NCBI登录号NM_001034819）存在差异。与参考基因相比，对照肉鸡显示出四个核苷酸差异（c.192A>G、c.519C>T、903G>A和960C>G），而对照蛋鸡在c.33G>C、192A>G、858G>A、904G>A、960C>G和1257C>T处存在差异，总共六个差异。然而，在对照肉鸡和对照蛋鸡品系之间，在c.33G>C、519T>C、858G>A、903A>G、904G>A和1257C>T处观察到核苷酸差异。参考基因与对照肉鸡之间氨基酸水平的变化是p.D320N，与对照蛋鸡之间是p.D302N和p.D320N。另一方面，在对照肉鸡和对照蛋鸡品系之间观察到一个氨基酸差异（p.D302N）。系统发育研究显示肉鸡和蛋鸡品系与参考基因以及其他鸟类物种之间关系密切，形成了一个聚类。这些聚类反过来与哺乳动物物种显示出较远的联系。BMP 3基因的表达谱在胚胎发育的不同阶段以及品系的胚后期均表现出差异，对照蛋鸡的表达高于肉鸡。通过蛋白质印迹法在肉鸡和蛋鸡品系的骨髓组织中也检测到了该蛋白。得出的结论是，BMP 3基因序列在品系之间的核苷酸和氨基酸水平上存在差异，并且该基因在胚胎发育的不同时期以及孵化后期表达存在差异。
