Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Department of Food Science, University of Copenhagen, Copenhagen, Denmark.
Diabetes Metab Res Rev. 2018 Sep;34(6):e3010. doi: 10.1002/dmrr.3010. Epub 2018 May 1.
Oral insulin as a preventive strategy and/or treatment of type 1 diabetes has been the target of much research. Producing oral insulins is a complex and challenging task, with numerous pitfalls, due to physiological, physical, and biochemical barriers. Our aim was to determine the impact of oral insulin on the delicate gut microbiota composition.
Female nonobese diabetic mice were given oral porcine insulin 2 times a week from 5 weeks of age for 4 weeks, and then subsequently once a week for 21 weeks, or until euthanized. The mice were divided into groups on a gluten-reduced diet or a standard diet. Gut microbiota composition was analysed based on faecal samples, and the type 1 diabetes incidence of the mice was monitored.
We observed no influence of the oral porcine insulin on the gut microbiota composition of mice on a gluten-reduced or a standard diet at 9 weeks of age. Also, the administration of oral insulin did not influence the incidence of type 1 diabetes at 30 weeks of age.
Oral porcine insulin does not alter the gut microbiota composition of nonobese diabetic mice on either a gluten-reduced diet or standard diet. Also, the oral porcine insulin did not influence the incidence of type 1 diabetes in the groups.
口服胰岛素作为预防策略和/或治疗 1 型糖尿病一直是许多研究的目标。由于生理、物理和生化屏障，生产口服胰岛素是一项复杂且具有挑战性的任务，存在许多陷阱。我们的目的是确定口服胰岛素对肠道微生物群组成的影响。
从 5 周龄开始，每周给雌性非肥胖型糖尿病（NOD）小鼠口服猪胰岛素 2 次，持续 4 周，然后每周 1 次，持续 21 周，或直至处死。将小鼠分为谷朊粉减少饮食组或标准饮食组。根据粪便样本分析肠道微生物群组成，并监测小鼠 1 型糖尿病的发病率。
我们观察到，在 9 周龄时，口服猪胰岛素对谷朊粉减少饮食或标准饮食的小鼠肠道微生物群组成没有影响。此外，口服胰岛素的给予也没有影响 30 周龄时 1 型糖尿病的发病率。
口服猪胰岛素不会改变谷朊粉减少饮食或标准饮食的 NOD 小鼠的肠道微生物群组成。此外，口服猪胰岛素也不会影响各组 1 型糖尿病的发病率。
