Department of Chemical Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Department of Textile Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
J Biomed Mater Res A. 2018 Sep;106(9):2394-2401. doi: 10.1002/jbm.a.36431. Epub 2018 May 3.
In the present research, a ternary polycaprolactone (PCL)/gelatin/fibrinogen nanofibrous scaffold for tissue engineering application was developed. Through this combination, PCL improved the scaffold mechanical properties; meanwhile, gelatin and fibrinogen provided more hydrophilicity and cell proliferation. Three types of nanofibrous scaffolds containing different fibrinogen contents were prepared and characterized. Morphological study of the nanofibers showed that the prepared nanofibers were smooth, uniform without any formation of beads with a significant reduction in nanofiber diameter after incorporation of fibrinogen. The chemical characterization of the scaffolds confirmed that no chemical reaction occurred between the scaffold components. The tensile test results of the scaffolds showed that increasing in fibrinogen content led to a decrease in mechanical properties. Furthermore, adipose-derived stem cells were employed to evaluate cell-scaffold interaction. Cell culture results indicated that higher cell proliferation occurred for the higher amount of fibrinogen. Statistical analysis was also carried out to evaluate the significant difference for the obtained results of water droplet contact angle and cell culture. Therefore, the results confirmed that PCL/gel/fibrinogen scaffold has a good potential for tissue engineering applications including central nerve system tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2394-2401, 2018.
在本研究中，开发了一种用于组织工程应用的三元聚己内酯（PCL）/明胶/纤维蛋白原纳米纤维支架。通过这种组合，PCL 提高了支架的机械性能；同时，明胶和纤维蛋白原提供了更好的亲水性和细胞增殖能力。制备并表征了含有不同纤维蛋白原含量的三种类型的纳米纤维支架。纳米纤维的形态研究表明，所制备的纳米纤维光滑、均匀，没有任何珠粒形成，在掺入纤维蛋白原后纳米纤维直径显著减小。支架的化学表征证实了支架成分之间没有发生化学反应。支架的拉伸试验结果表明，纤维蛋白原含量的增加导致机械性能下降。此外，还使用脂肪源性干细胞来评估细胞-支架相互作用。细胞培养结果表明，较高的纤维蛋白原含量会导致更高的细胞增殖。还对水接触角和细胞培养的结果进行了统计学分析，以评估获得结果的显著性差异。因此，结果证实 PCL/明胶/纤维蛋白原支架具有很好的组织工程应用潜力，包括中枢神经系统组织工程。© 2018 Wiley Periodicals, Inc. J 生物材料 Res 部分 A:106A:2394-2401, 2018.
