Department of Dentistry, Kut University College, Kut, Wasit, 52001, Iraq.
College of Technical Engineering, The Islamic University, Najaf, Iraq.
Bioprocess Biosyst Eng. 2022 Jun;45(6):981-997. doi: 10.1007/s00449-022-02717-9. Epub 2022 Apr 9.
In this study, blend nanofibrous scaffolds were electrospun from polycaprolactone/gelatin (PCL/Gel) blend solutions reinforced by bone morphogenetic protein (BMP)-modified graphene oxide (GO). SEM results showed that uniform and bead-less nanofibers with 270 nm average diameter were obtained from electrospun of PCL/Gel blend solutions. Tensile strength test and contact angle measurement demonstrated that addition of PCL led to higher mechanical and physical properties of the resulting nanofibers. The addition of PCL as well as GO in the blend supports the suitable mechanical strength in the body media. The loading of BMP-modified graphene in the Gel/PCL structure caused the formation of nanofibrous substrate with great resemblance to bone tissue. Gel/PCL-G hybrid nanofibers revealed good biocompatibility in the presence of human osteosarcoma cells, and no trace of cellular toxicity was observed. The cells grown on the scaffolds exhibited a spindle-like and broad morphology and almost uniformly covered the entire nanofiber scaffold. Gel/PCL nanofibers reinforced by graphene oxide-immobilized bone morphogenetic protein was prepared as a promising safe and biocompatible nanofiber with high antibacterial activity for bone tissue engineering.
在这项研究中，混合纳米纤维支架由聚己内酯/明胶（PCL/Gel）共混溶液通过电纺而成，该共混溶液中添加了骨形态发生蛋白（BMP）修饰的氧化石墨烯（GO）。SEM 结果表明，从 PCL/Gel 共混溶液的电纺中获得了具有 270nm 平均直径的均匀且无珠的纳米纤维。拉伸强度测试和接触角测量表明，添加 PCL 导致所得纳米纤维具有更高的机械和物理性能。PCL 和 GO 的添加支持在体内介质中具有合适的机械强度。在 Gel/PCL 结构中加载 BMP 修饰的石墨烯导致形成与骨组织非常相似的纳米纤维基底。在存在人骨肉瘤细胞的情况下，Gel/PCL-G 杂化纳米纤维显示出良好的生物相容性，没有观察到细胞毒性的痕迹。在支架上生长的细胞呈梭形和宽形形态，几乎均匀地覆盖整个纳米纤维支架。通过固定骨形态发生蛋白的氧化石墨烯增强的 Gel/PCL 纳米纤维被制备为一种有前途的安全且生物相容的纳米纤维，具有用于骨组织工程的高抗菌活性。
