Allafchian Ali Reza, Jalali Seyed Amir Hossein, Mousavi Seyed Ebrahim
Research Institute for Nanotechnology and Advanced Materials, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
IET Nanobiotechnol. 2018 Dec;12(8):1108-1113. doi: 10.1049/iet-nbt.2018.5071.
In this study, a polymer obtained from the basil seed mucilage (BSM) in combination with polycaprolactone (PCL), was used in the 2D scaffold production process for cell culture. First, combinations of two polymers with different ratios and concentrations were prepared and electrospun. Among these samples, a sample with a BSM/PCL ratio of 2/3 was used to perform different tests due to its fibre uniformity and appropriate diameter. The Fourier transform infrared spectrometer test was carried out to chemically analyse the scaffold, the X-ray diffraction test to determine the crystallinity of the scaffold, and the contact angle test to determine the hydrophilicity of the scaffold. The strength, porosity, and degradation percentage of the scaffold were also studied. With appropriate conditions of the scaffold for cell culture determined, Vero epithelial cells were cultured on the scaffold. Results obtained from cell culture indicated that the adhesion of the scaffold was suitable for the appropriate growth cells.
在本研究中，一种由罗勒籽粘液（BSM）与聚己内酯（PCL）结合而成的聚合物被用于二维支架的细胞培养生产过程。首先，制备了不同比例和浓度的两种聚合物组合并进行静电纺丝。在这些样品中，由于其纤维均匀性和合适的直径，使用了BSM/PCL比例为2/3的样品进行不同测试。进行了傅里叶变换红外光谱仪测试以对支架进行化学分析，进行了X射线衍射测试以确定支架的结晶度，并进行了接触角测试以确定支架的亲水性。还研究了支架的强度、孔隙率和降解百分比。在确定了适合细胞培养的支架条件后，将Vero上皮细胞培养在该支架上。细胞培养获得的结果表明，该支架的粘附性适合细胞的适当生长。
