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用于生物医学应用的辣根过氧化物酶催化交联使明胶基电纺纤维不溶解

Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications.

作者信息

Furuno Kotoko, Wang Jing, Suzuki Keiichiro, Nakahata Masaki, Sakai Shinji

机构信息

Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.

Institute for Advanced Co-Creation Studies, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.

出版信息

ACS Omega. 2020 Aug 13;5(33):21254-21259. doi: 10.1021/acsomega.0c03164. eCollection 2020 Aug 25.

DOI:10.1021/acsomega.0c03164
PMID:32875262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7450611/
Abstract

Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells.

摘要

通过静电纺丝含有带酚羟基（Ph）基团的明胶（明胶-Ph）和辣根过氧化物酶（HRP）的水溶液，获得了直径为160纳米的水不溶性明胶基纳米纤维。通过将静电纺丝的纳米纤维暴露于含有过氧化氢的空气中，HRP催化Ph基团的交联反应，从而实现纳米纤维的水不溶性。静电纺丝纳米纤维中的HRP活性为天然HRP的65%。水不溶性明胶-Ph纳米纤维在组织工程应用中所需的细胞相容性通过人胚胎肾源HEK293细胞的粘附和活力得到证实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/1d10a8ac4dd8/ao0c03164_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/a33d4202680c/ao0c03164_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/3a94cae2ad86/ao0c03164_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/e1bb40f18a08/ao0c03164_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/0f81e066e805/ao0c03164_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/e25d0ebee82d/ao0c03164_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/1d10a8ac4dd8/ao0c03164_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/a33d4202680c/ao0c03164_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/3a94cae2ad86/ao0c03164_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/e1bb40f18a08/ao0c03164_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/0f81e066e805/ao0c03164_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/e25d0ebee82d/ao0c03164_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9826/7450611/1d10a8ac4dd8/ao0c03164_0007.jpg

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