Malekpoor Fatemeh, Salimi Azam, Pirbalouti Abdollah Ghasemi
Acta Pol Pharm. 2016 Sep;73(5):1229-1234.
Basil (Ocimum basilicun L.) belongs to the family Lamiaceae and is an important aromatic and medicinal plant, which it widely cultivated in many countries. This plant is a good source of phenolic com- pounds and natural antioxidants. The main aim of present study was to determine effect of jasmonic acid on total phenolic content and antioxidant activity of ethanolic extract from the aerial parts of the purple and green landraces of basil. The pot experiment was conducted in an experimental field with cold and semiarid climate in southwestern Iran. Treatments comprised control (water), ethanol as solvent, 200 and 400 pLjasmonic acid. The total phenolic content of the extract by Folin-Ciocalteu method and the antioxidant activity using DPPH assay were determined. Results indicated that the different levels of jasmonic acid had significant effects on total phenolics content and antioxidant activity of the extracts. Foliar-applied jasmonic acid in particular 400 μL increased total phenolic content in the plants as compared to untreated plants. In conclusion, it is suggested that jasmonic acid as an abiotic elicitor could be a promising material used to increase biological activity and pro-health functional value of basil plants.
罗勒（Ocimum basilicum L.）属于唇形科，是一种重要的芳香和药用植物，在许多国家广泛种植。这种植物是酚类化合物和天然抗氧化剂的良好来源。本研究的主要目的是确定茉莉酸对紫色和绿色地方品种罗勒地上部分乙醇提取物的总酚含量和抗氧化活性的影响。盆栽试验在伊朗西南部寒冷半干旱气候的试验田进行。处理包括对照（水）、乙醇作为溶剂、200和400微升茉莉酸。采用福林-酚法测定提取物的总酚含量，采用DPPH法测定抗氧化活性。结果表明，不同水平的茉莉酸对提取物的总酚含量和抗氧化活性有显著影响。与未处理的植株相比，叶面喷施茉莉酸尤其是400微升可增加植株的总酚含量。总之，建议茉莉酸作为一种非生物诱导剂，可能是一种有前途的材料，用于提高罗勒植株的生物活性和促进健康的功能价值。
