Sharma Anket, Kumar Vinod, Yuan Huwei, Kanwar Mukesh Kumar, Bhardwaj Renu, Thukral Ashwani Kumar, Zheng Bingsong
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India.
Front Plant Sci. 2018 Nov 2;9:1609. doi: 10.3389/fpls.2018.01609. eCollection 2018.
The present study focused on assessing the effects of jasmonic acid (JA) seed treatment on the physiology of seedlings grown under imidacloprid (IMI) toxicity. It has been observed that IMI application declined the chlorophyll content and growth of seedlings. However, JA seed treatment resulted in the significant recovery of chlorophyll content and seedling growth. Contents of oxidative stress markers like superoxide anion, hydrogen peroxide, and malondialdehyde were enhanced with IMI application, but JA seed treatment significantly reduced their contents. Antioxidative defense system was activated with IMI application which was further triggered after JA seed treatment. Activities of antioxidative enzymes and contents of non-enzymatic antioxidants were enhanced with the application of IMI as well as JA seed treatment. JA seed treatment also regulated the gene expression of various enzymes under IMI stress. These enzymes included respiratory burst oxidase (), Ribulose-1,5-bisphosphate carboxylase/oxygenase (), NADH-ubiquinone oxidoreductase (), carboxylesterase (), chlorophyllase (), cytochrome P450 monooxygenase (). JA seed treatment up-regulated the expressions of , and under IMI toxicity. However, expressions of and were down-regulated in seedlings germinated from JA seed treatment and grown in presence of IMI. Seed soaking with JA also resulted in a significant reduction of IMI residues in seedlings. The present study concluded that seed soaking with JA could efficiently reduce the IMI toxicity by triggering the IMI detoxification system in intact plants.
本研究着重评估茉莉酸（JA）种子处理对在吡虫啉（IMI）毒性下生长的幼苗生理的影响。据观察，施用IMI会降低幼苗的叶绿素含量和生长。然而，JA种子处理使叶绿素含量和幼苗生长显著恢复。施用IMI会提高超氧阴离子、过氧化氢和丙二醛等氧化应激标志物的含量，但JA种子处理显著降低了它们的含量。施用IMI激活了抗氧化防御系统，JA种子处理后该系统进一步被触发。施用IMI以及JA种子处理均提高了抗氧化酶的活性和非酶抗氧化剂的含量。JA种子处理还调节IMI胁迫下各种酶的基因表达。这些酶包括呼吸爆发氧化酶、1,5-二磷酸核酮糖羧化酶/加氧酶、NADH-泛醌氧化还原酶、羧酸酯酶、叶绿素酶、细胞色素P450单加氧酶。在IMI毒性下，JA种子处理上调了、 和 的表达。然而，在经JA种子处理萌发并在IMI存在下生长的幼苗中， 和 的表达下调。用JA浸种还显著降低了 幼苗中IMI的残留量。本研究得出结论，用JA浸种可通过触发完整植物中的IMI解毒系统有效降低IMI毒性。
