Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry Fruit Trees, Plant Science and Technology College, Beijing University of Agriculture, Beijing 102202, China.
Beijing Bei Nong Enterprise Management Co., Ltd., Beijing 102206, China.
Int J Mol Sci. 2018 Apr 11;19(4):1165. doi: 10.3390/ijms19041165.
The present study aimed to compare polyphenols among red lettuce cultivars and identify suitable cultivars for the development and utilization of healthy vegetables. Polyphenols, mineral elements, and antioxidant activity were analyzed in the leaves of six red pigmented lettuce ( L.) cultivars; thereafter, we assessed the anti-tumor effects of cultivar B-2, which displayed the highest antioxidant activity. Quadrupole-Orbitrap mass spectrometry analysis revealed four classes of polyphenols in these cultivars. The composition and contents of these metabolites varied significantly among cultivars and primarily depended on leaf color. The B-2 cultivar had the highest antioxidant potential than others because it contained the highest levels of polyphenols, especially anthocyanin, flavone, and phenolic acid; furthermore, this cultivar displayed anti-tumor effects against the human lung adenocarcinoma cell line A549, human hepatoma cell line Bel7402, human cancer colorectal adenoma cell line HCT-8, and HT-29 human colon cancer cell line. Hence, the new red-leaf lettuce cultivar B-2 has a distinct metabolite profile, with high potential for development and utilization of natural phytochemical and mineral resources in lettuces and can be used as a nutrient-dense food product.
本研究旨在比较红色生菜品种中的多酚含量，并确定适合开发和利用健康蔬菜的品种。分析了 6 个红色色素生菜（L.）品种叶片中的多酚、矿物质元素和抗氧化活性；然后，我们评估了抗氧化活性最高的品种 B-2 的抗肿瘤作用。四极杆轨道阱质谱分析显示，这些品种中存在四类多酚。这些代谢物的组成和含量在品种间差异显著，主要取决于叶片颜色。B-2 品种的抗氧化潜力最高，因为它含有最高水平的多酚，特别是花青素、黄酮和酚酸；此外，该品种对人肺腺癌细胞系 A549、人肝癌细胞系 Bel7402、人结直肠腺瘤细胞系 HCT-8 和人结肠癌细胞系 HT-29 均显示出抗肿瘤作用。因此，新型红色生菜品种 B-2 具有独特的代谢物特征，具有开发和利用生菜中天然植物化学物质和矿物质资源的巨大潜力，可作为营养密集型食品。
