Department of Animal and Rural Technology, State University of Southwest Bahia (UESB), Itapetinga, Bahia, Brazil.
Department of Exact and Technological Sciences, State University of Santa Cruz (UESC), Ilhéus, Bahia, Brazil.
PLoS One. 2018 Apr 11;13(4):e0195206. doi: 10.1371/journal.pone.0195206. eCollection 2018.
The use of cocoa pod husk hemicellulose hydrolysate (CPHHH) was evaluated for the production of xylitol by Candida boidinii XM02G yeast isolated from soil of cocoa-growing areas and decaying bark, as an alternative means of reusing this type of waste. Xylitol was obtained in concentrations of 11.34 g.L-1, corresponding to a yield (Yp/s) of 0.52 g.g-1 with a fermentation efficiency (ε) of 56.6%. The yeast was tolerant to inhibitor compounds present in CPHHH without detoxification in different concentration factors, and was able to tolerate phenolic compounds at approximately 6 g.L-1. The yeast was also able to metabolize more than 99% (p/v) of furfural and hydroxymethylfurfural present in the non-detoxified CPHHH without extension of the cell-growth lag phase, showing the potential of this microorganism for the production of xylitol. The fermentation of cocoa pod husk hydrolysates appears to provide an alternative use which may reduce the impact generated by incorrect disposal of this waste.
利用可可荚壳半纤维素水解物（CPHHH）生产木糖醇，使用的是从可可种植区土壤和腐烂树皮中分离出的毕赤酵母（Candida boidinii XM02G），这是一种再利用这种废物的替代方法。在不同的浓缩因子下，酵母可以耐受 CPHHH 中存在的抑制剂化合物而无需解毒，并且可以耐受约 6 g·L-1 的酚类化合物。酵母还能够代谢未经解毒的 CPHHH 中存在的超过 99%（v/v）的糠醛和羟甲基糠醛，而不会延长细胞生长的迟滞期，这表明该微生物在生产木糖醇方面具有潜力。可可荚壳水解物的发酵似乎提供了一种替代用途，可以减少这种废物不当处理产生的影响。
