Magalhães Patrícia Künzle Ribeiro, Miranda Carlos Henrique, Vilar Fernando Crivelenti, Schmidt André, Bittar Roberta Rodrigues, Paixão Giselle Aparecida Caixe de Carvalho, Martinez Edson Zangiacomi, Maciel Léa Maria Zanini
Divisão de Endocrinologia, Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirão Preto, SP, Brasil.
Departamento de Medicina Social, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirão Preto, SP, Brasil.
Arch Endocrinol Metab. 2018 Apr 5;62(2):201-204. doi: 10.20945/2359-3997000000026. Print 2018 Mar-Apr.
To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening.
Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage.
The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer.
Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.
评估样本干燥及储存温度对新生儿筛查中促甲状腺激素（TSH）稳定性的影响。
采集29名成年志愿者的血样作为新生儿血样的替代样本（10名TSH正常，9名显性甲状腺功能减退，10名亚临床甲状腺功能减退），滴于滤纸上，在22°C或35°C干燥3小时。然后将样本分别储存在22°C、-4°C或-20°C，在储存的第0天（D0）、第7天、第30天、第60天、第180天和第360天进行TSH检测。
干燥温度在D0时不影响TSH检测。储存在22°C时TSH值在D30前保持稳定，储存在冰箱或冰柜中时TSH值在D60前保持稳定。储存在22°C的样本TSH值下降幅度大于储存在冰箱或冰柜中的样本。
与储存在冰箱中相比，冷冻储存并无优势。在一年结束时，如果需要对初始结果进行确认，应考虑TSH浓度的降低。
