Aral Cüneyt A, Köseoğlu Serhat, Sağlam Mehmet, Pekbağrıyanık Tuğba, Savran Levent
Department of Periodontology, Faculty of Dentistry, Sifa University, İzmir, 35100, Turkey.
Department of Periodontology, Faculty of Dentistry, İzmir Katip Çelebi University, İzmir, Turkey.
Inflammation. 2016 Jun;39(3):986-93. doi: 10.1007/s10753-016-0328-0.
Periostin, an extracellular matrix protein functioning as an important structural mediator and adhesion molecule, has been shown to be an important regulator of connective tissue integrity. This study aimed to evaluate the levels of periostin in chronic periodontitis (CP) and aggressive periodontitis (AgP) compared to non-periodontitis (NP). Individuals were submitted to gingival crevicular fluid (GCF) and saliva sampling. Periodontal examination consisted of plaque index (PI), gingival index (GI), probing depth (PD), bleeding on probing (BOP), and clinical attachment level (CAL) measurements. Assays for periostin were performed by an enzyme-linked immunosorbent assay. Periodontitis patients presented more severe clinical indices compared to the NP group (p < 0.001). The mean GCF level of periostin was lowest in the AgP group as compared to the other groups and was lower in the CP group as compared to the NP group (p < 0.001). Increased levels of periostin were observed in the saliva of patients with AgP as compared to the CP and NP groups (p < 0.05). There was a negative relationship between GCF periostin levels and clinical parameters (p < 0.01), whereas a positive correlation was observed between salivary periostin levels and full-mouth GI and CAL scores (p < 0.01). To our knowledge, this is the first report investigating periostin levels in GCF and saliva in aggressive periodontitis. The results suggest that subjects with CP and AgP exhibit a different periostin profile. Periostin in GCF may have a protective role against periodontal disease. Furthermore, salivary periostin concentrations may have a promising diagnostic potential for the aggressive forms of periodontal disease.
骨膜蛋白是一种细胞外基质蛋白，作为重要的结构介质和黏附分子，已被证明是结缔组织完整性的重要调节因子。本研究旨在评估与非牙周炎（NP）相比，慢性牙周炎（CP）和侵袭性牙周炎（AgP）中骨膜蛋白的水平。研究对象接受龈沟液（GCF）和唾液采样。牙周检查包括菌斑指数（PI）、牙龈指数（GI）、探诊深度（PD）、探诊出血（BOP）和临床附着水平（CAL）测量。采用酶联免疫吸附测定法检测骨膜蛋白。与NP组相比，牙周炎患者的临床指标更严重（p < 0.001）。与其他组相比，AgP组的GCF骨膜蛋白平均水平最低，与NP组相比，CP组的该水平较低（p < 0.001）。与CP组和NP组相比，AgP患者唾液中的骨膜蛋白水平升高（p < 0.05）。GCF骨膜蛋白水平与临床参数之间呈负相关（p < 0.01），而唾液骨膜蛋白水平与全口GI和CAL评分之间呈正相关（p < 0.01）。据我们所知，这是第一份研究侵袭性牙周炎中GCF和唾液中骨膜蛋白水平的报告。结果表明，CP和AgP患者表现出不同的骨膜蛋白谱。GCF中的骨膜蛋白可能对牙周疾病具有保护作用。此外，唾液骨膜蛋白浓度可能对侵袭性牙周疾病具有潜在的诊断价值。
