Kozbor D, Tripputi P, Roder J C, Croce C M
J Immunol. 1984 Dec;133(6):3001-5.
We produced somatic cell hybrids between human myeloma cells and a lymphoblastoid cell line that is hypoxanthine phosphoribosyl transferase-deficient and ouabain-resistant. These hybrids were phenotypically similar to the human myeloma parental cells and grew as well as the human lymphoblastoid parental cells. After counterselection in 6-thioguanine, mutants that were 6-thioguanine-and ouabain-resistant were obtained, one of which was used as a fusion partner with lymphoblastoid B cells that produce anti-tetanus toxoid (TT) antibodies. These hybrids secreted human anti-TT monoclonal antibodies in much larger amounts than the parental lymphoblastoid cells, and were stable for a period of over 10 mo until the present time. Thus, by hybridizing plasmacytomas with lymphoblastoid cells, we constructed a fusion partner that secretes large amounts of immunoglobulin (Ig), grows at a fast rate, has a high fusion frequency, and supports the production of monoclonal antibodies over long periods of time. Moreover, anti-TT antibody-producing hybrids have been grown as solid tumors in irradiated BALB/c nude mice and then adopted to ascites growth, producing 1 to 8 mg of human immunoglobulin per 1 ml of ascites fluid.
我们制备了人骨髓瘤细胞与一种次黄嘌呤磷酸核糖基转移酶缺陷且对哇巴因耐药的淋巴母细胞系之间的体细胞杂种。这些杂种在表型上与亲本骨髓瘤细胞相似，生长情况与亲本淋巴母细胞一样良好。在6-硫鸟嘌呤中进行反选择后，获得了对6-硫鸟嘌呤和哇巴因耐药的突变体，其中一个被用作与产生抗破伤风类毒素（TT）抗体的淋巴母细胞B细胞的融合伙伴。这些杂种分泌的人抗TT单克隆抗体比亲本淋巴母细胞多得多，并且直到现在在超过10个月的时间内都保持稳定。因此，通过将浆细胞瘤与淋巴母细胞杂交，我们构建了一个能分泌大量免疫球蛋白（Ig）、生长迅速、融合频率高且能长期支持单克隆抗体产生的融合伙伴。此外，产生抗TT抗体的杂种已在经辐照的BALB/c裸鼠中长成实体瘤，然后转为腹水生长，每1毫升腹水中产生1至8毫克人免疫球蛋白。
