Department of Dermatology, Dong Guan People's Hospital, Guangdong Province, Dongguan 523018, People's Republic of China.
Department of Gynaecology and Obstetrics, Dong Guan People's Hospital, Guangdong Province, Dongguan 523018, People's Republic of China.
Dermatol Ther. 2018 May;31(3):e12598. doi: 10.1111/dth.12598. Epub 2018 Apr 11.
This study aims to explore the expression of RORγt, BATF, and IL-17 in Chinese vitiligo patients with 308 nm excimer laser treatment. One hundred and sixty-four vitiligo patients treated with 308 nm excimer laser were enrolled as Case group and 137 health examiners as Control group. Quantitative real-time polymerase chain reaction and immunohistochemistry were conducted to detect the expressions of RORγt, BATF, and IL-17. Expression of RORγt, BATF, IL-17A, and IL-17F were higher in Case group than Control group, with the diagnostic accuracy of 88.04, 87.38, 97.34, and 89.04%, respectively. Pearson correlation analysis showed a positive correlation in RORγt, BATF, IL-17A, and IL-17F mRNAs in vitiligo patients. Moreover, their expressions were higher in active vitiligo patients than stable ones. Besides, the expressions of RORγt, BATF, IL-17A, and IL-17F in vitiligo skin were significantly higher than those in non lesional skin and normal controls. After treatment, their expressions were significantly decreased. Active vitiligo and the high expressions of RORγt, BATF, and IL-17F were the independent risk factors for the ineffectiveness of 308 nm excimer laser treatment. The expressions of RORγt, BATF, IL-17 were significantly enhanced in vitiligo patients, which were correlated with the activity of vitiligo and 308 nm excimer laser therapeutic effects.
本研究旨在探讨 RORγt、BATF 和 IL-17 在接受 308nm 准分子激光治疗的中国白癜风患者中的表达。将 164 例接受 308nm 准分子激光治疗的白癜风患者纳入病例组，137 例健康体检者纳入对照组。采用实时荧光定量聚合酶链反应和免疫组织化学法检测 RORγt、BATF 和 IL-17 的表达。结果显示，病例组 RORγt、BATF、IL-17A 和 IL-17F 的表达均高于对照组，其诊断准确率分别为 88.04%、87.38%、97.34%和 89.04%。Pearson 相关性分析显示，白癜风患者 RORγt、BATF、IL-17A 和 IL-17FmRNA 呈正相关。此外，活动期白癜风患者的表达高于稳定期患者。此外，白癜风患者皮损中 RORγt、BATF、IL-17A 和 IL-17F 的表达明显高于非皮损和正常对照组。治疗后，其表达明显降低。活动期白癜风和 RORγt、BATF、IL-17F 的高表达是 308nm 准分子激光治疗无效的独立危险因素。白癜风患者 RORγt、BATF、IL-17 的表达明显增强，与白癜风的活动度及 308nm 准分子激光治疗效果相关。
