Cytochrome P450 CYP6EV11 in Chironomus kiiensis Larvae Involved in Phenol Stress.

Phenol is one of the organic pollutants which can cause water environment pollution. It is not only enriched in aquatic organisms but is also a serious threat to human health. is very sensitive to the contaminants in water and its cytochrome P450s are usually chosen as biomarkers for water pollution. To examine whether plays a role in the oxidative metabolism of phenol, we measured the silencing efficiency of and evaluated larval susceptibility to sublethal phenol levels by RNA interference (RNAi) technology. The results showed that the transcription of was found significantly up-regulated when the 4th instar larvae were exposed to three doses of phenol. However, the transcriptional levels of were significantly suppressed by 92.7% in the 4th instar larvae soaked in compared with those soaked in for 6 h. The expression and mortality of the 4th instar larvae with silencing were mostly decreased under phenol stress. Therefore, the gene may be used as a molecular biomarker for earlier warning and monitoring for water pollution.