Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Ecotoxicol Environ Saf. 2023 Sep 15;263:115353. doi: 10.1016/j.ecoenv.2023.115353. Epub 2023 Aug 14.
UDP-glucuronosyltransferases (UGTs) could transform various exogenous and endogenous compounds, which help detoxification of pesticides in insects. To investigate the role of UGTs in the detoxification metabolism of insecticides in Chironomus kiiensis, CkUGT302M1, CkUGT302N1, CkUGT308N1 and CkUGT36J1 genes were identified with 1449-1599 bp encoding 482-532 amino acids. Four UGT genes shared 40.86∼53.36% identity with other homologous insect species, and expressed in all developmental stages, notably in the larval and adult stages. Expression of CkUGTs was higher in the gastric caecum, midgut and head. Moreover, CkUGTs expression and activity were significantly increased in C. kiiensis larvae in exposure to sublethal concentrations of carbaryl, deltamethrin and phoxim, respectively. To further explore the functions of UGT genes, the CkUGT308N1 was effectively silenced in 4th instar C. kiiensis larvae by RNA interference, which resulted in the mortality of dsCkUGT308N1 treated larvae increased by 71.43%, 111.11% and 62.50% under sublethal doses of carbaryl, deltamethrin and phoxim at the 24-h time point, respectively. The study revealed that the CkUGT308N1 gene in C. kiiensis could contribute to the metabolism of pesticides and provide a scientific basis for evaluating the water pollution of pesticides.
尿苷二磷酸葡萄糖醛酸转移酶（UGTs）可以转化各种外源性和内源性化合物，有助于昆虫中农药的解毒。为了研究 UGTs 在奇隆斯库蚊（Chironomus kiiensis）杀虫剂解毒代谢中的作用，鉴定了 CkUGT302M1、CkUGT302N1、CkUGT308N1 和 CkUGT36J1 基因，它们分别编码 482-532 个氨基酸。这四个 UGT 基因与其他同源昆虫物种的同源性为 40.86∼53.36%，在所有发育阶段都有表达，特别是在幼虫和成虫阶段。CkUGTs 在胃盲囊、中肠和头部的表达量较高。此外，在亚致死浓度的甲萘威、溴氰菊酯和辛硫磷暴露下，奇隆斯库蚊幼虫的 CkUGTs 表达和活性显著增加。为了进一步探索 UGT 基因的功能，通过 RNA 干扰有效地沉默了第 4 龄奇隆斯库蚊幼虫中的 CkUGT308N1，结果表明，dsCkUGT308N1 处理的幼虫在亚致死剂量的甲萘威、溴氰菊酯和辛硫磷作用 24 小时后，死亡率分别增加了 71.43%、111.11%和 62.50%。该研究表明，奇隆斯库蚊中的 CkUGT308N1 基因可能有助于农药的代谢，并为评估农药的水污染提供了科学依据。
