Prakash Om, Kumar Amit, Sonwane Arvind, Rathore Rajesh, Singh Ran Vir, Chauhan Anuj, Kumar Pushpendra, Renjith R, Yadav Ramji, Bhaladhare Ashish, Baqir Mohd, Sharma Deepak
Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India.
Mol Biol Rep. 2014 May;41(5):2815-25. doi: 10.1007/s11033-014-3136-3. Epub 2014 Jan 28.
Genetic susceptibility to brucellosis is multifactorial, and it is known that impairment of the immune system could contribute to risk for getting brucellosis. The aim of the study was to find association of bovine brucellosis with 20 SNPs pertaining to bovine cytokine (IFNG, IFNGR1, IFNGR2, TNFA) and innate immunity (SLC11A1, TLR1, TLR4, and TLR9) genes using PCR-RFLP genotyping technique and it was observed that SLC11A1 (+1066 C/G), TLR1 (+1446 C/A), TLR1 (+1380 G/A), TLR4 (+10 C/T) and TLR4 (+399 C/T) loci were significantly (P≤0.05) associated with bovine brucellosis. The odds ratios (OR) of CG and CC genotypes versus GG genotype were 0.31 (0.12-0.82; 95% CI) and 0.18 (0.03-1.06; 95% CI) at SLC11A1 (+1066 C/G) locus in cases of brucellosis affected cattle. For TLR1 (+1380 G/A) locus, the OR for AG and AA genotypes versus GG genotypes were 0.15 (0.05-0.44; 95% CI) and 0.26 (0.04-1.47; 95% CI) which indicated that proportion of GG homozygote was significantly higher in brucellosis affected animals as compared to control. At TLR1 (+1446 C/A) locus the OR of AC genotype versus CC genotype was 0.24 (0.08-0.68; 95% CI) which revealed that relative proportion CC genotypes was significantly higher in case population. The TLR4 (+10 C/T) locus had three genotypes (TT, CT and CC) where OR of CT and CC genotypes versus TT genotype were near to zero. The OR of CT genotypes versus CC genotypes was 8.25 (0.94-71.92; 95% CI) at TLR4 (+399 C/T) locus and indicated that CT genotype had higher odds of bovine brucellosis than control animals.
布鲁氏菌病的遗传易感性是多因素的，并且已知免疫系统受损可能会增加感染布鲁氏菌病的风险。本研究的目的是使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）基因分型技术，寻找与牛细胞因子（IFNG、IFNGR1、IFNGR2、TNFA）和固有免疫（SLC11A1、TLR1、TLR4和TLR9）基因相关的20个单核苷酸多态性（SNP）与牛布鲁氏菌病的关联。研究发现，SLC11A1（+1066 C/G）、TLR1（+1446 C/A）、TLR1（+1380 G/A）、TLR4（+10 C/T）和TLR4（+399 C/T）位点与牛布鲁氏菌病显著相关（P≤0.05）。在患布鲁氏菌病的牛中，SLC11A1（+1066 C/G）位点的CG和CC基因型与GG基因型的比值比（OR）分别为0.31（0.12 - 0.82；95%置信区间）和0.18（0.03 - 1.06；95%置信区间）。对于TLR1（+1380 G/A）位点，AG和AA基因型与GG基因型的OR分别为0.15（0.05 - 0.44；95%置信区间）和0.26（0.04 - 1.47；95%置信区间），这表明与对照组相比，患布鲁氏菌病动物中GG纯合子的比例显著更高。在TLR1（+1446 C/A）位点，AC基因型与CC基因型的OR为0.24（0.08 - 0.68；95%置信区间），这表明病例组中CC基因型的相对比例显著更高。TLR4（+10 C/T）位点有三种基因型（TT、CT和CC），其中CT和CC基因型与TT基因型的OR接近零。在TLR4（+399 C/T）位点，CT基因型与CC基因型的OR为8.25（0.94 - 71.92；95%置信区间），这表明CT基因型患牛布鲁氏菌病的几率高于对照动物。
