Selvam Ramasamy, Saravanakumar Marimuthu, Suresh Subramaniyam, Chandrasekeran C V, Prashanth D'Souza
Animal Health Science, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India.
R&D Biology, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India.
Asian-Australas J Anim Sci. 2018 Nov;31(11):1795-1806. doi: 10.5713/ajas.18.0018. Epub 2018 Apr 12.
The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC).
A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined.
Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities.
Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.
本研究旨在建立肉鸡胆碱缺乏模型（CDM），以评估多草药配方（PHF）与合成氯化胆碱（SCC）相比的功效。
在三个实验中，将总共2550只1日龄的科宝430肉鸡随机分配到不同组。在实验1中，G1和G2作为正常对照组，分别饲喂以100%豆粕（SBM）为主要蛋白质来源且添加和不添加SCC的基础日粮。在G3、G4、G5和G6组中，用大豆分离蛋白（SPI）分别替代25%、50%、75%和100%的SBM，以诱导不同程度的胆碱缺乏。在实验2中，评估了PHF（500和1000克/吨）与SCC（1000克/吨）的效果。在实验3中，确定了PHF（200、400和500克/吨）与SCC（400克/吨）的剂量反应。
用SPI替代SBM导致体重增加（BWG）呈线性下降，饲料转化率（FCR）不佳。用SPI替代25%的SBM对BWG和FCR产生了最佳负面影响；因此，将其用于进一步研究。在实验2中，PHF（500和1000克/吨）和SCC（1000克/吨）在BWG、FCR和相对肝脏重量方面表现相似。在实验3中，PHF在400克/吨时产生了最佳效果，在恢复血清天冬氨酸转氨酶活性、腹部脂肪、胸肌脂质含量和肝脏组织病理学异常方面与SCC相当。
用SPI替代SBM导致胆碱缺乏，其特征为BWG恶化、FCR变差、肝酶升高以及表明脂肪肝的组织病理学变化。发现CDM可有效评估SCC和PHF。得出的结论是，PHF有潜力通过恢复CDM造成的负面影响来模拟SCC的生物活性。
