Marimuthu Saravanakumar, Suresh Subramaniyam, D'Souza Prashanth
R & D Centre, Natural Remedies Private Limited, Bengaluru, India.
J Adv Vet Anim Res. 2022 Sep 30;9(3):432-439. doi: 10.5455/javar.2022.i611. eCollection 2022 Sep.
To elucidate the cellular mechanisms of polyherbal formulation [Kolin Plus (KP)], genomics was performed to delineate the genes and pathways associated with lipid regulation through transcriptional profiling of the liver in commercial broilers raised on diets deficient in choline chloride (CCL).
The gene expression patterns were studied for four groups [normal diet: normal, choline chloride deficient (CCD), KP (400 gm/ton), and CCL (400 gm/ton)] using Agilent microarray on day 42. The hierarchical cluster analysis was carried out on 12,614 differentially expressed genes (DEGs) with a similar expression.
Out of 12,614 significant DEGs, 1,926, 448, and 1,330 genes were expressed at higher rates, and 413, 482, and 1,364 were expressed at lower rates than CCD (CCD normal), CCL (CCL CCD), and KP (KP CCD), respectively. GO enrichment analysis of DEG further revealed the significant association of biological process items with the lipid, sterol, and lipoprotein metabolic processes. In particular, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, carnitine palmitoyl transferase I, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta, and patatin-like phospholipase domain containing 2 genes involved in fatty acid oxidation and lipase C, ABCG5, ABCG8, acetyl-CoA carboxylase, ATP citrate lyase enzyme, and peroxisome proliferator-activated receptor gamma genes involved in lipogenesis were altered by KP intervention for lipid metabolism.
These findings reveal that the supplementation of KP prevents fatty liver-associated problems in broiler chickens by modulating the expression of the above-mentioned genes that are responsible for the oxidation of fatty acids and lipogenesis in the liver.
为阐明多草药配方[科林加（KP）]的细胞机制，通过对缺乏氯化胆碱（CCL）日粮饲养的商品肉鸡肝脏进行转录谱分析，开展基因组学研究以确定与脂质调节相关的基因和通路。
在第42天，使用安捷伦微阵列对四组[正常日粮：正常组、氯化胆碱缺乏（CCD）组、KP（400克/吨）组和CCL（400克/吨）组]的基因表达模式进行研究。对12,614个表达相似的差异表达基因（DEG）进行层次聚类分析。
在12,614个显著的DEG中，与CCD组（CCD vs正常组）、CCL组（CCL vs CCD组）和KP组（KP vs CCD组）相比，分别有1,926、448和1,330个基因表达率较高，413、482和1,364个基因表达率较低。DEG的基因本体（GO）富集分析进一步揭示了生物过程条目与脂质、固醇和脂蛋白代谢过程的显著关联。特别是，参与脂肪酸氧化的过氧化物酶体增殖物激活受体γ共激活因子1α、肉碱棕榈酰转移酶I、羟酰基辅酶A脱氢酶三功能多酶复合物亚基β和含patatin样磷脂酶结构域2基因，以及参与脂肪生成的脂肪酶C、ABCG5、ABCG8、乙酰辅酶A羧化酶、ATP柠檬酸裂解酶和过氧化物酶体增殖物激活受体γ基因，在脂质代谢方面因KP干预而发生改变。
这些发现表明，补充KP可通过调节上述负责肝脏脂肪酸氧化和脂肪生成的基因表达，预防肉鸡脂肪肝相关问题。
