Zhou Xin, Zhang Lei, Fu Shi-Jie, Liu Gang, Guo Xiao-Guang, Yi Gang, Liu Yang, Wang Guo-You
1Department of Orthopedics, Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou 646000, China; 2Academician Workstation in Luzhou,Luzhou 646000,China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Mar 20;38(3):346-352. doi: 10.3969/j.issn.1673-4254.2018.03.17.
To study the effect of Shaoyangzhugu (SYZG) Formula (a formula consisting of 9 traditional Chinese drugs) in delaying the degeneration of articular cartilage and the role p19-p53-p21 signaling pathway in mediating this effect.
Thirteen aged cynomolgus monkeys with degenerative knee joints were selected based on X-ray findings, and one of them was randomly selected for pathological observation. The other monkeys were randomized equally into SYZG Formula group (treated with SYZG decoction), ammonia moxime group and saline group. All the monkeys were sacrificed after 8 weeks of treatment with intragastric administration of the drugs or saline. The pathology in the knee joint articular cartilage was observed and the mRNA and protein expressions of p19, p53, and p21 in the articular cartilage were detected using RT-qPCR and Western blotting.
The pathological findings of the articular cartilage in old cynomolgus monkeys were consistent with the characteristics of knee osteoarthritis (KOA). Mankin scores of the cynomolgus monkeys were 7.38∓0.52 in SYZG Formula group, 7.88∓0.83 in ammonia moxime group, and 8.38∓0.74 in saline group, showing a significant difference between SYZG Formula group and saline group (P<0.05). The expressions of p19, p53, and p21 were the lowest in SYZG Formula group and the highest in saline group with significant differences among the 3 groups (P<0.05).
SYZG Formula can delay chondrocyte senescence by regulating p19-p53-p21 signaling pathway to delay articular cartilage degeneration in aged cynomolgus monkeys.
研究少阳逐骨（SYZG）方（由9味中药组成的方剂）延缓关节软骨退变的作用及p19-p53-p21信号通路在介导该作用中的作用。
根据X线检查结果选取13只膝关节退变的老年食蟹猴，随机选取1只进行病理观察。其余猴子随机等分为SYZG方组（给予SYZG水煎剂治疗）、氨甲环酸组和生理盐水组。所有猴子经灌胃给予药物或生理盐水治疗8周后处死。观察膝关节软骨病理变化，采用RT-qPCR和Western blotting检测软骨中p19、p53和p21的mRNA及蛋白表达。
老年食蟹猴关节软骨病理表现符合膝骨关节炎（KOA）特征。SYZG方组食蟹猴Mankin评分7.38±0.52，氨甲环酸组7.88±0.83，生理盐水组8.38±0.74，SYZG方组与生理盐水组比较差异有统计学意义（P<0.05）。p19、p53和p21表达在SYZG方组最低，生理盐水组最高，3组间差异有统计学意义（P<0.05）。
SYZG方可通过调节p19-p53-p21信号通路延缓老年食蟹猴关节软骨退变，从而延缓软骨细胞衰老。
