Nalwa Aasma, Walia Ritika, Singh Varsha, Madan Karan, Mathur Sandeep, Iyer Venkateshwaran, Jain Deepali
Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India.
J Cytol. 2018 Apr-Jun;35(2):94-98. doi: 10.4103/JOC.JOC_248_16.
Liquid-based cytology (LBC), initially developed for screening gynecologic specimens, is now being used in nongynecologic aspiration and exfoliative specimens. In this study, the diagnostic yield and utility of thin-prep (TP) was compared with conventional preparations to ascertain its utility in improving the diagnosis of respiratory lesions.
Bronchial washings (BW) and transbronchial needle aspirates (TBNA) (bronchoscopy/endobronchial ultrasound-guided) from 70 consecutive patients of mediastinal masses and endo/peribronchial growths were included. The diagnostic yields of both conventional smears and thin-prep were compared. Immunocytochemistry (ICC) was performed on direct/cytospin smears of TBNA/BW and TP slides when the tumor could not be subtyped by morphology. Histopathologic correlation was done.
Although well-preserved morphological features and cleaner background in TP allowed accurate diagnosis of malignancies, diagnostic yield was comparable to conventional preparations. Immunocytochemistry was successfully employed on TP smears which helped in accurate subtyping of the tumors. Few shortcomings of TP smears were uneven distribution of cells, thick cell clusters, and inadequate cellularity.
Liquid-based TP preparation is an effective diagnostic tool for respiratory tract cytology, however, results are comparable to conventional smears.
液基细胞学（LBC）最初是为筛查妇科标本而开发的，现在正用于非妇科的穿刺和脱落标本。在本研究中，将薄层制片（TP）与传统制片的诊断率和实用性进行比较，以确定其在改善呼吸道病变诊断方面的效用。
纳入70例连续的纵隔肿块和支气管内/支气管周围肿物患者的支气管冲洗液（BW）和经支气管针吸活检（TBNA）（支气管镜/支气管内超声引导）样本。比较传统涂片和薄层制片的诊断率。当肿瘤无法通过形态学进行亚型分类时，对TBNA/BW的直接涂片/细胞离心涂片和TP玻片进行免疫细胞化学（ICC）检测，并进行组织病理学对照。
尽管TP制片中细胞形态保存良好且背景更清晰，有助于准确诊断恶性肿瘤，但其诊断率与传统制片相当。TP涂片上成功进行了免疫细胞化学检测，有助于准确对肿瘤进行亚型分类。TP涂片存在一些缺点，如细胞分布不均、细胞团块厚和细胞数量不足。
液基TP制片是呼吸道细胞学的一种有效诊断工具，然而，其结果与传统涂片相当。
