Brown Kevin M, Long Shaojun, Sibley L David
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Bio Protoc. 2018 Feb 20;8(4). doi: 10.21769/BioProtoc.2728.
is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding 's 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are needed to functionally investigate hundreds of putative essential protein coding genes. Accordingly, we recently implemented the auxin-inducible degron (AID) system for studying essential proteins in . Here we provide a step-by-step protocol for examining protein function in using the AID system in a tissue culture setting.
是被称为顶复门的致命原生动物寄生虫门的成员。作为一种典型的顶复门生物，关于其8000多个蛋白质编码基因有大量信息，包括序列变异、表达以及对寄生虫适应性的相对贡献。然而，需要新的工具来对数百个推定的必需蛋白质编码基因进行功能研究。因此，我们最近实施了生长素诱导降解子（AID）系统来研究中的必需蛋白质。在这里，我们提供了一个在组织培养环境中使用AID系统研究中蛋白质功能的逐步方案。
